EGF Protein

Catalog No. ABIN2452188

Product Details

Target: EGF (Epidermal Growth Factor (EGF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Characteristics: Activity: The ED50 as determined by a cell proliferation assay using MTS assay kit (CellTiter 96 AQueous Assay, Promega) with NHEK cells (normal human epidermal keratinocytes) was < 0.1 ng/mL, corresponding to a specific activity of < 1 x 107 units/mg.
The full-length human EGF was expressed in E.coli and highly purified by the propriety chromatographic procedures. This product has the same amino acid sequence with the mature human EGF (6.35 kDa) and has no tag attached.

Purity: > 95 % as determined by SDS-PAGE (CBB staining)

Sterility: Sterile filtered

Application Details

Application Notes: 1. EGF is commonly used as a supplement in serum-free or reduced serum media for culture of mammalian cells.
2. Studies of the human EGF receptor,transmembrane signaling and protein phosphorylation
3. Western blotting control for anti-EGF antibodies
4. Acceleration of the healing process of epidermis damage on skin,cornea,etc.
5. Widely used in cosmetics,such as whitening,anti-wrinkle,anti-aging,etc.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 10 mM Na-phosphate ( pH 7.2), 50 % glycerol

Storage: -20 °C/-80 °C

Storage Comment: -20 C (For long term storage: -70 C)

References

Carpenter, Cohen: "Epidermal growth factor." in: The Journal of biological chemistry, Vol. 265, Issue 14, pp. 7709-12, (1990) (PubMed).

Cohen, Carpenter: "Human epidermal growth factor: isolation and chemical and biological properties." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, Issue 4, pp. 1317-21, (1975) (PubMed).

Target Details

Target: EGF (Epidermal Growth Factor (EGF))

Abstract: EGF Products

Synonyms: HOMG4 Protein, URG Protein, AI790464 Protein, CEGF Protein, epidermal growth factor Protein, pro-epidermal growth factor Protein, EGF Protein, egf Protein, CpipJ_CPIJ020278 Protein, Egf Protein

Background: EGF is a polypeptide growth hormone which plays an important role in the regulation of growth, proliferation, and differentiation of a wide range of cells by binding to its receptor EGFR (epidermal growth factor receptor). Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways.

Gene ID: 1950

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation