Pertussis Toxin Protein (ARTC)

Catalog No. ABIN2452202

Product Details

Target: Pertussis Toxin (ARTC)

Protein Type: Native

Biological Activity: Active

Origin: Bordetella pertussis

Source: Bordetella pertussis

Application: Functional Studies (Func)

Characteristics: This product was highly purified (>90 % pure) from Bordetella pertussis strain Tohama by the method of Skelton & Wong. Cytotoxicity of the PT was confirmed by morphological alteration of CHO cells after treatment with 0.1 ng/mL of PT.

Application Details

Application Notes: 1. Studies on the signal transduction of the trimeric GTP binding proteins.
2. Therapeutic role in treating a number of diseases including hypertention,viral and autoimumune inhibitions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 83 µg/mL

Buffer: 10 % glycerol, 10 mM phoshate buffer ( pH 7. 4), 137 mM NaCl

Storage: -80 °C

References

Skelton, Wong: "Simple, efficient purification of filamentous hemagglutinin and pertussis toxin from Bordetella pertussis by hydrophobic and affinity interaction." in: Journal of clinical microbiology, Vol. 28, Issue 5, pp. 1062-5, (1990) (PubMed).

Target Details

Target: Pertussis Toxin (ARTC)

Alternative Name: Pertussis Toxin (ARTC Products)

Background: Perrtussis toxin (PT) is a protein-based AB5-type exotoxin produced by Bordeterra pertussis. PT catalyzes the ADP-ribosylation of the alpha subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt and prevents intracellular signal transduction involving the G proteins. PT consists of one moplecule of each S1 (26 kDa), S2 (22 kDa), S3 (22 kDa), S5 (12 kDa) and two molecule of S4 (12 kDa).