ATP1B1 Protein

Catalog No. ABIN5326718

Product Details

Target: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Reactivity: Please inquire

Host: Please inquire

Application: Western Blotting (WB)

Brand: SERVA®

Application Details

Application Notes: Standard curve: 3 loads are recommended (0.5, 2 and 4i1/4l).For most applications a sample load of 0.2i1/4g of chlorophyll will give a AtpB signal in this range.Positive control: load per well: a 2i1/4l load is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10 % glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Comment:

Concentration: after adding 225 μl of dest. water final concentration of the standard is 0.27 pmol/μl.Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For reconstitution add 225 μL of milliQ water

Buffer: Glycerol 10 %, Tris Base 141 mM, Tris HCl 106 mM, LDS 2 %, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/mL PefaBloc protease inhibitor (Roche), 50mM DTT.

Handling Advice: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References

Johnson: "Altered expression of the chloroplast ATP synthase through site-directed mutagenesis in Chlamydomonas reinhardtii." in: Photosynthesis research, Vol. 96, Issue 2, pp. 153-62, (2008) (PubMed).

Target Details

Target: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Alternative Name: AtpB (AtpB) (ATP1B1 Products)

Synonyms: ATP1B Protein, Atp4b Protein, Atpb Protein, Atpb-1 Protein, NKbeta1 Protein, ATPBS Protein, atp1b1 Protein, atp1b1a Protein, cb710 Protein, ATPase Na+/K+ transporting subunit beta 1 Protein, ATPase, Na+/K+ transporting, beta 1 polypeptide Protein, ATPase beta chain Protein, ATP synthase CF1 beta subunit Protein, ATPase Na+/K+ transporting subunit beta 1 S homeolog Protein, ATPase, Na+/K+ transporting, beta 1b polypeptide Protein, ATP1B1 Protein, Atp1b1 Protein, atpB Protein, atp1b1.S Protein, atp1b1b Protein

Background: ATP synthase is the universal enzyme that stnthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.This product is a recombinant protein standard, source: Synechocystis strain PCC 6803.

Molecular Weight: 50-54 kDa

Pathways: Thyroid Hormone Synthesis, Ribonucleoside Biosynthetic Process, SARS-CoV-2 Protein Interactome