NOG Protein (AA 28-232, N-Term)

Catalog No. ABIN2666486

Product Details

Target: NOG (Noggin (NOG))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 28-232, N-Term

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Endotoxin Level:

Less than 0.1 ng per μg of protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Mouse Noggin is able to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC5 chondrogenic cells with an ED50 of 1.0 - 2.0 ng/ml, corresponding to a specific activity of 5.0 x 105 - 1.0 x 106 units/mg.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. Note: Due to solubility reasons the protein should be kept at low pH . It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -20°C or -70°C.

Target Details

Target: NOG (Noggin (NOG))

Alternative Name: Noggin (NOG Products)

Synonyms: SYM1 Protein, SYNS1 Protein, nog-A Protein, nog1 Protein, noggin-1 Protein, noggin Protein, noggin Protein, noggin L homeolog Protein, noggin protein Protein, NOG Protein, Nog Protein, nog.L Protein, noggin Protein

Background: Noggin is an embryonic inducer that can sequester TGF-β cytokines of the BMP family with extremely high affinity. This protein was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Noggin has also been shown to modulate the activities of other BMPs including BMP-2, -7, -13, and -14. It is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle, and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons. Noggin also appears to maintain adult stem cell populations in vivo. For example, it maintains neural stem cells within the hippocampus. Targeted deletion of Noggin in mice results in prenatal death and a recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis.

Molecular Weight: Recombinant Mouse Noggin is a 46.4 kDa disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains. The predicted N-terminal amino acid is Met.

Pathways: Stem Cell Maintenance, Tube Formation