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GZMB Protein (AA 17-247)

GZMB Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % , as determined by Coomassie stained SDS-PAGE. FACS Active
Catalog No. ABIN2666523
  • Target See all GZMB Proteins
    GZMB (Granzyme B (GZMB))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 17-247
    Origin
    • 10
    • 7
    • 3
    • 1
    • 1
    Mouse
    Source
    • 8
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    HEK-293 Cells
    Application
    Flow Cytometry (FACS)
    Purity
    > 95 % , as determined by Coomassie stained SDS-PAGE.
    Sterility
    0.22 μm filtered
    Endotoxin Level

    Less than 1.0 EU per μg of protein as determine by the LAL method.

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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Biological activity: Recombinant mouse Granzyme B supplied in its inactive form. Once the mouse Granzyme B is activated by active mouse Capthepsin C/DPPI, it is able to cleave the peptide substrate t-Butyloxycaronyl-Ala-Ala-ThioBenzyl ester (Boc-AAD-SBzl) in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB)1,2, with an activity > 3000 pmol/min/μg.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    For maximum results, quick spin vial prior to opening. The recombinant protein could be aliquoted and stored at -20 °C in a sterile buffer (20 mM Tris, 150 mM NaCl, and pH 7.5).
    Concentration
    200 μg/mL
    Buffer
    0.22 μm filtered protein solution is in 20 mM Tris, 150 mM NaCl, and pH 7.5.
    Handling Advice
    Avoid repeated freeze/thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Unopened vial can be stored at -70°C for six months.
  • Target
    GZMB (Granzyme B (GZMB))
    Alternative Name
    Granzyme B (GZMB Products)
    Synonyms
    CCPI Protein, CGL-1 Protein, CGL1 Protein, CSP-B Protein, CSPB Protein, CTLA1 Protein, CTSGL1 Protein, HLP Protein, SECT Protein, granzyme B Protein, GZMB Protein
    Background
    Granzyme B is a serine protease expressed by cytotoxic T cells (CTL) and NK cells. Its main function is to induce cell death to eliminate harmful targets such as allogeneic, virally infected, and tumorous cells. This is evident by the fact that CTLs from mice deficient of granzyme B exhibit a profound defect in inducing rapid DNA fragmentation and apoptosis in target cells. Following receptor-mediated conjugate formation between CTL or NK and their target cell, granzyme B enters the target via endocytosis, and subsequently activates multiple protein substrates to induce apoptosis. Most circulating CD56+ CD8- NK cells, and approximately half of circulating CD8+ T cells, coexpress both granzyme A and B. In contrast, few circulating CD4+ T cells express granzymes A or B. Activation of CD8+ and CD4+ T cells induces substantial expression of granzyme B, but not granzyme A. Besides CTL and NK, evidence has shown that the distribution of human granzyme B has a broader spectrum of cells including CD34+ hematopoietic progenitor cells, keratinocytes, basophils, mast cells, plasmacytoid dendritic cells, and B cells. Although its role in cytotoxic lymphocyte-mediated apoptosis is well established, granzyme B can also degrade extracellular matrix proteins and alter inflammation if present in the extracellular milieu. These findings suggest that granzyme B can function as an activation molecule with potentially important immunoregulatory functions. In addition, it was shown that expression of granzyme B is elevated in acute coronary syndrome and acute myocardia infarction, indicating that granzyme B could be a factor involved in cardiovascular diseases.
    Molecular Weight
    This 246 amino acid recombinant protein has a predicted molecular mass of approximately 27.5 kDa. The protein migrates at approximately 37 kDa in DTT-reducing conditions and approximately 37 kDa in non-reducing conditions by SDS-PAGE. The predicted N-term
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis
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