Collagen, Type XVIII, alpha 1 (COL18A1) (AA 1154-1335), (N-Term) (Active) Protein

Details for Product No. ABIN2666547, Supplier: Log in to see
Protein Name
  • kno
  • kno1
  • cb371
  • cb529
  • fc96d06
  • sb:cb529
  • wu:fb47h10
  • wu:fb77g12
  • wu:fc50f04
  • wu:fc96d06
  • wu:fk02h12
  • KNO
  • KNO1
  • KS
  • collagen, type XVIII, alpha 1
  • collagen type XVIII, alpha 1
  • COL18A1
  • col18a1
  • Col18a1
Protein Characteristics
AA 1154-1335, N-Term
3
1
1
1
1
1
Origin
Human
15
3
1
Source
Escherichia coli (E. coli)
10
3
2
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Western Blotting (WB)
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Purity > 95 % , as determined by Coomassie stained SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Background Endostatin is a C-terminal proteolytic fragment of collagen XVIII α1 (Col 18) that is localized in the vascular basement membrane of almost all epithelia and endothelia. Endostatin is produced from catalytic cleavage from MMPs, cathepsin L, and S. It is detected in circulation at a physiologic level of 20 to 50 ng/mL in serum. Crystal X-ray analysis of endostatin has shown a compact globular structure, with one face rich in arginine residues that acts as a heparin-binding motif. Endostatin interacts with multiple molecules including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate, thrombospondin-1, SPARC, collagens (I, IV, and VI), the amyloid peptide Aβ (1-42), transglutaminase-2, and among others. Endostatins derived from collagens XV and XVIII differ in structure and binding properties, tissue distribution, and anti-angiogenic activity. Endostatin is an endogenous angiostatic factor that induces apoptosis in endothelial cells and inhibits endothelial cell migration in vitro. Col 18-null mice showed faster tumor growth when implanted with tumor cells that do not produce Col 18. Overexpression of circulating endostatin in transgenic mice resulted in reduced tumor growth. Experimental model of glomerulonephritis in Col 18-null mice showed that the absence of this molecule exacerbates immune-mediated glomerulonephritis.
Molecular Weight The 192 amino acid recombinant protein has a predicted molecular mass of approximately 21.3 kDa. The DTT-reduced and non-reduced protein migrate at approximately 23 and 20 kDa respectively by SDS-PAGE. The predicted N-terminal amino acid is Met.
Research Area Cancer, Angiogenesis, Extracellular Matrix, Hormones
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Biological activity: ED50 = 0.5 - 3 μg/ml, as determined by inhibition of HUVEC cell proliferation induced by human FGF basic.

Restrictions For Research Use only
Format Liquid
Reconstitution For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
Buffer 0.22 μm filtered protein solution is in PBS, pH 7.2.
Handling Advice Avoid repeated freeze/thaw cycles.
Storage -20 °C
Storage Comment Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
Supplier Images
ELISA image for Collagen, Type XVIII, alpha 1 (COL18A1) (AA 1154-1335), (N-Term) (Active) Protein (ABIN2666547) Collagen, Type XVIII, alpha 1 (COL18A1) (AA 1154-1335), (N-Term) (Active) protein
Background publications Veillard, Saidi, Burden, Scott, Gillet, Lecaille, Lalmanach: "Cysteine cathepsins S and L modulate anti-angiogenic activities of human endostatin." in: The Journal of biological chemistry, Vol. 286, Issue 43, pp. 37158-67, 2011 (PubMed).

Hamano, Okude, Shirai, Sato, Kimura, Ogawa, Ueda, Yokosuka, Kalluri, Ueda: "Lack of collagen XVIII/endostatin exacerbates immune-mediated glomerulonephritis." in: Journal of the American Society of Nephrology : JASN, Vol. 21, Issue 9, pp. 1445-55, 2010 (PubMed).

Faye, Chautard, Olsen, Ricard-Blum: "The first draft of the endostatin interaction network." in: The Journal of biological chemistry, Vol. 284, Issue 33, pp. 22041-7, 2009 (PubMed).

Sund, Hamano, Sugimoto, Sudhakar, Soubasakos, Yerramalla, Benjamin, Lawler, Kieran, Shah, Kalluri: "Function of endogenous inhibitors of angiogenesis as endothelium-specific tumor suppressors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 8, pp. 2934-9, 2005 (PubMed).

Zatterstrom, Felbor, Fukai, Olsen: "Collagen XVIII/endostatin structure and functional role in angiogenesis." in: Cell structure and function, Vol. 25, Issue 2, pp. 97-101, 2000 (PubMed).