LGALS1/Galectin 1 Protein (AA 2-135, N-Term)

Catalog No. ABIN2666816

Product Details

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 2-135, N-Term

Origin: Human

Source: CHO Cells

Application: Flow Cytometry (FACS)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Sterility: 0.22 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Recombinant human Galectin-1 is able to agglutinate human red blood cells with an ED50 of 0.2 - 1.0 μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 0.3M NaCl, 2 mM β-mercaptoethanol, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Alternative Name: Galectin-1 (LGALS1 Products)

Synonyms: GAL1 Protein, GBP Protein, Galectin-1 Protein, LGAS1 Protein, LGALS1 Protein, MGC64502 Protein, GB10026 Protein, AA410090 Protein, Gal-1 Protein, Galbp Protein, L-14.5 Protein, L14 Protein, Lect14 Protein, galectin-1 Protein, Galaptin Protein, galectin 1 Protein, galectin-1 Protein, lectin, galactoside-binding, soluble, 1 L homeolog Protein, protein enabled Protein, lectin, galactose binding, soluble 1 Protein, LGALS1 Protein, Lgals1 Protein, CC1G_05003 Protein, CC1G_05004 Protein, lgals1.L Protein, LOC408848 Protein

Background: Galectin-1 is a 14 kD lectin widely expressed in mammals and produced by vascular, interstitial, epithelial, and immune cells. This protein binds specifically to galactoside derivatives of glycoproteins on cell membranes. A great number of membrane glycoconjugates have been identified as binding partners of Galectin-1 such as β1 integrins, CD2, CD3, CD4, CD43, CD45, and GM1 ganglioside. Galectin-1 also binds to a number of extracellular-matrix components, such as laminin and fibronectin in a dose-dependent and β-galactoside-dependent manner. Because Galectin-1 is a divalent lectin, it can also form complexes with receptor partners participating in cell-matrix recognition. Thus, Galectin-1 is involved in a variety of biological activities such as cell-matrix interactions, immune responses, inflammatory responses, tumorigenesis, and apoptosis of both tumor and immune cells. In addition, Galectin-1 is thought to play a role in creating immune tolerance in pregnancy. Galectin-1 induces the differentiation of dendritic cells towards a phenotype which dampens T helper 1 cells and T helper 17 cells and dampens inflammation via IL-10 and IL-27. It also plays a role in the formation and expression of HLA-G in the syncytium. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation.

Molecular Weight: This 155 amino acid recombinant protein has a predicted molecular mass of approximately 16.8 kDa. The protein migrates at approximately 24 kDa by SDS-PAGE in DTT-reducing conditions. The N-terminal amino acid is His.

Pathways: Carbohydrate Homeostasis