Neuregulin 1 (NRG1) (AA 176-246) (Active) Protein

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Protein Name
  • NRG1
  • wu:fm70e01
  • DKFZp459A1838
  • HPRG
  • HRGP
  • THPH11
  • HRG1
  • ARIA
  • GGF
  • GGF2
  • HGL
  • HRG
  • HRGA
  • MST131
  • NDF
  • SMDF
  • 6030402G23Rik
  • D230005F13Rik
  • GGFII
  • HRGalpha
  • Hgl
  • pro-neuregulin-1
  • NRG-1
  • heregulin
  • nrg1-A
  • neuregulin 1
  • histidine-rich glycoprotein
  • NRG1
  • nrg1
  • Nrg1
  • HRG
  • Hrg
Protein Characteristics
AA 176-246
5
5
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Origin
Human
37
8
5
3
1
Source
Escherichia coli (E. coli)
27
15
6
2
2
1
Protein Type
Recombinant
Biological Activity
Active
Application
Flow Cytometry (FACS)
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Available images

Purity > 95 % , as determined by Coomassie stained SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Background Neuregulin 1 (NRG1) was initially identified in the conditioned medium of a human breast tumor cell line as a protein of 45 kD, which induces the phosphorylation of a tyrosine kinase (ERBB2). The neuregulin family includes four members (NRG1-NRG4) that are encoded from four individual genes. Through differential promoter usage and splicing, these members generate 12 isoforms. The best characterized is neuregulin 1, which includes eight isoforms, all isoforms contain (from N-terminus to C-terminus) an immunoglobulin-like (Ig) domain, a growth factor-like domain (EGF), a transmembrane (TM), and a cytoplasmic domain. Alternative splicing in the EGF-like domain of neuregulin 1 results in α and β isoforms. The EGF-like domain is necessary and sufficient for neuregulin bioactivity. The EGFR family of receptors includes four members (ErbB1 through ErbB4), and neuregulins bind to the ErbB3 and ErbB4 receptors. Neuregulins are released from the membrane by proteolytic cleavage, and this process is required for the binding of the neuregulin derived fragments to ErbB receptors. The neuregulin1-β isoform is predominant in the central nervous system and participates in development, survival, and metabolism in neuron and glial cells. Neuregulin1-β is neuroprotective and attenuates inflammatory responses induced by ischemic stroke in rats, preventing macrophage and microglial infiltration and astrocytic activation. Also, neuregulin1-β blocks the induction of pro-inflammatory and stress genes provoked by ischemia. Neuregulins 1-4 are expressed in approximately 25 % of breast cancer carcinomas and increase breast cancer cell proliferation, increase tumorigenesis, and promote invasive characteristics of cancer cells. In this sense, neuregulin1-β induces MMP-1 and MMP-9 in cancer cell lines.
Molecular Weight The 71 amino acid recombinant protein has a predicted molecular mass of approximately 8.2 kDa. The DTT-reduced and non-reduced protein migrate at approximately 11 kDa and 12 kDa respectively by SDS-PAGE. The predicted N-terminal amino acid is Thr.
Research Area Neurology, Glia marker
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Biological activity: ED50 = 0.2 - 1 ng/ml, corresponding to a specific activity of 1 - 5 x 106 units/mg, as determined by induction of MCF-7 cell proliferation.

Restrictions For Research Use only
Format Liquid
Reconstitution For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
Buffer 0.22 μm filtered protein solution is in PBS, pH 7.2.
Handling Advice Avoid repeated freeze/thaw cycles.
Storage -20 °C
Storage Comment Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
Supplier Images
ELISA image for Neuregulin 1 (NRG1) (AA 176-246) (Active) Protein (ABIN2666853) Neuregulin 1 (NRG1) (AA 176-246) (Active) protein
Background publications Zhang, Wong, Duan, Jacobs, Borden, Bedogni: "An ERBB3/ERBB2 oncogenic unit plays a key role in NRG1 signaling and melanoma cell growth and survival." in: Pigment cell & melanoma research, Vol. 26, Issue 3, pp. 408-14, 2013 (PubMed).

Luo, Prior, He, Hu, Tang, Shen, Yadav, Kiryu-Seo, Miller, Trapp, Yan: "Cleavage of neuregulin-1 by BACE1 or ADAM10 protein produces differential effects on myelination." in: The Journal of biological chemistry, Vol. 286, Issue 27, pp. 23967-74, 2011 (PubMed).

Shamir, Buonanno: "Molecular and cellular characterization of Neuregulin-1 type IV isoforms." in: Journal of neurochemistry, Vol. 113, Issue 5, pp. 1163-76, 2010 (PubMed).

Buac, Xu, Cronin, Weeraratna, Hewitt, Pavan: "NRG1 / ERBB3 signaling in melanocyte development and melanoma: inhibition of differentiation and promotion of proliferation." in: Pigment cell & melanoma research, Vol. 22, Issue 6, pp. 773-84, 2009 (PubMed).

Mei, Xiong: "Neuregulin 1 in neural development, synaptic plasticity and schizophrenia." in: Nature reviews. Neuroscience, Vol. 9, Issue 6, pp. 437-52, 2008 (PubMed).

Tan, Wang, Gold, Chen, Dean, Harrison, Weinberger, Law: "Molecular cloning of a brain-specific, developmentally regulated neuregulin 1 (NRG1) isoform and identification of a functional promoter variant associated with schizophrenia." in: The Journal of biological chemistry, Vol. 282, Issue 33, pp. 24343-51, 2007 (PubMed).

Holmes, Sliwkowski, Akita, Henzel, Lee, Park, Yansura, Abadi, Raab, Lewis: "Identification of heregulin, a specific activator of p185erbB2." in: Science (New York, N.Y.), Vol. 256, Issue 5060, pp. 1205-10, 1992 (PubMed).