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CCL20 Protein (Ala27, Met96)

CCL20 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 98 % , as determined by Coomassie stained SDS-PAGE. FACS Active
Catalog No. ABIN2666889
  • Target See all CCL20 Proteins
    CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    Ala27, Met96
    Origin
    • 17
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    Human
    Source
    • 29
    • 2
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Application
    Flow Cytometry (FACS)
    Purity
    > 98 % , as determined by Coomassie stained SDS-PAGE.
    Sterility
    0.22 μm filtered
    Endotoxin Level

    Less than 0.01 ng per μg cytokine as determined by the LAL method.

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    Discover our top product CCL20 Protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Biological activity: Bioactivity was measured by its property to chemoattract BaF3-hCCR6 transfectants in a dose dependent manner.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
    Buffer
    0.22 μm filtered protein solution is in PBS.
    Handling Advice
    Avoid repeated freeze/thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
  • Target
    CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
    Alternative Name
    CCL20 (CCL20 Products)
    Synonyms
    MIP-3ALPHA Protein, CCL20 Protein, si:dkey-150o13.1 Protein, CKb4 Protein, LARC Protein, MIP-3-alpha Protein, MIP-3a Protein, MIP3A Protein, SCYA20 Protein, ST38 Protein, MIP-3A Protein, MIP-3[a] Protein, Scya20 Protein, exodus-1 Protein, C-C motif chemokine ligand 20 Protein, chemokine (C-C motif) ligand 20b Protein, chemokine (C-C motif) ligand 20 Protein, CCL20 Protein, ccl20b Protein, Ccl20 Protein
    Background
    During infections, CCL20 expression is stimulated in epithelial cells, and immature CCR6+ DC are recruited. Thereafter, CCR6 is downregulated during dendritic cell (DC) maturation, and these migrate to the lymphoid node under the expression of CCR7. CCL20 is the only ligand for CCR6. Nevertheless, mouse and human β-defensin-1 and -2, small cationic antimicrobial peptides, bind and activate CCR6. Contrarily, these β-defensins have lower affinities for CCR6 than CCL20. Also, human CCL20 has potent antimicrobial activity. CCL20 and CCR6 play an important role in the pathogenesis of encephalomyelitis and psoriasis (chronic inflammatory skin disorder) in animal models. CCL20 is a chemokine abundant in psoriatic plaques characterized by epidermal hyperproliferation and immunocyte infiltration. CCL20 production from keratinocytes could be an initial trigger for Th17 activation and a key component in maintaining the self-perpetuating cycle of Th17 activation in psoriasis.
    Molecular Weight
    The 70 amino acid recombinant protein has a predicted molecular mass of approximately 8.0 kDa. The DTT-reduced protein migrates at approximately 11 kDa and non-reduced protein at approximately 13 kDa by SDS-PAGE. The N-terminal amino acid is Ala.
    Pathways
    The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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