BMP4 Protein (AA 303-408)

Catalog No. ABIN2667382

Product Details

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 303-408

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Purity: >98 % , as determined by Coomassie stained SDS-PAGE gel and HPLC analysis.

Endotoxin Level:

Less than 0.1 ng per μg of protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 = 5 - 10 ng/ml, corresponding to a specific activity of 1 - 2 x 105 units/mg, as measured by its ability to induce alkaline phosphatase production by ATDC-5 cells in a dose dependent manner.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in 5 mM sodium phosphate, pH 7.4 to a concentration of 0.1-1.0 mg/mL. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -20°C or -70°C.

Target Details

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Alternative Name: BMP-4 (BMP4 Products)

Synonyms: BMP2B Protein, BMP2B1 Protein, MCOPS6 Protein, OFC11 Protein, ZYME Protein, Bmp-4 Protein, Bmp2b Protein, Bmp2b-1 Protein, Bmp2b1 Protein, BOMPR4A Protein, bmp-4 Protein, zbmp-4 Protein, zgc:100779 Protein, BMP-4 Protein, XBMP-4 Protein, bmp2b Protein, bmp2b1 Protein, bmp4 Protein, ofc11 Protein, xbmp4 Protein, zyme Protein, BMP4 Protein, bone morphogenetic protein 4 Protein, bone morphogenetic protein 4 L homeolog Protein, bone morphogenetic protein 4 S homeolog Protein, BMP4 Protein, Bmp4 Protein, bmp4 Protein, bmp4.L Protein, bmp4.S Protein

Background: BMP-4 is a member of the bone morphogenetic protein (BMP) family of cytokines, which belong to the transforming growth factor beta (TGF-β) superfamily. BMP-4 plays a crucial function in the chondrogenic and osteogenic differentiation of mesenchymal stem cells as well as in the maintenance of adult cartilage and bone. Binding of BMP-4 to the BMPR-I/BMPR-II receptor complex was reported to activate several signaling pathways, such as the Smad, the MAPK, the PI3K/Akt, and the Wnt pathways, which are involved in bone formation, cell proliferation, and cell differentiation. Genetic defects in BMP-4 have been associated with microphthalmia and orofacial cleft.

Molecular Weight: The 106 amino acid recombinant protein has a predicted molecular mass of approximately 11.9 kDa. Recombinant BMP-4 is disulphide bond-linked homodimer. The predicted N-terminal amino acid is Lys.

Pathways: Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development