CNTF Protein (AA 2-200)
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- Target See all CNTF Proteins
- CNTF (Ciliary Neurotrophic Factor (CNTF))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 2-200
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Biochemical Assay (BCA)
- Purity
- >98 % , as determined by Coomassie stained SDS-PAGE.
- Endotoxin Level
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Less than 0.1 ng per μg of protein.
- Top Product
- Discover our top product CNTF Protein
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Ciliary Neurotrophic Factor (CNTF) (Active) protein
CNTF Origin: Rat Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His tag)CNTF Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His tag)CNTF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (GST tag)CNTF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Ciliary Neurotrophic Factor (CNTF) (AA 1-198) protein (Strep Tag)Crystallography grade CNTF Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 4-196), (partial) protein (His tag)CNTF Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Biological activity: ED50 = 50 - 150 ng/ml, corresponding to a specific activity of 0.67 - 2.0 x 104 units/mg, as measured by its ability to stimulate proliferation of human TF-1 cells in a dose dependent manner.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For maximum results, quick spin vial prior to opening. Reconstitute in water containing BSA (50 μg of BSA per 1 μg of protein) to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.
- Buffer
- Lyophilized, carrier-free.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Unopened vial can be stored at -20°C or -70°C.
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- Target
- CNTF (Ciliary Neurotrophic Factor (CNTF))
- Alternative Name
- CNTF (CNTF Products)
- Synonyms
- GPA Protein, CNTF Protein, HCNTF Protein, AI429687 Protein, ciliary neurotrophic factor Protein, CNTF Protein, Cntf Protein
- Background
- CNTF is a member of the four-helix bundle cytokine family and is structurally related to other family members such as IL-6, IL-11, and LIF. Binding of CNTF to its receptor CNTFRα triggers the association with the IL-6 receptor gp130 and the LIF receptor LIFRβ, followed by activating the JAK-STAT, the ERK, and the PI3K signaling pathways. CNTF was first identified as a neuroprotective factor that supported the survival of embryonic chick ciliary neurons. CNTF has been shown to regulate the differentiation and survival of a variety of neuronal cells and has been applied as the treatment for retinal degenerative diseases caused by genetic defect or damage induced by toxins, auto-antibodies, or strong light. CNTF is also revealed as a myotrophic factor for denervated skeletal muscle and a modulator for AMPK-mediated energy balance control.
- Molecular Weight
- The 199 amino acid recombinant protein has a predicted molecular mass of approximately 22.8 kDa. The predicted N-terminal amino acid is Ala.
- Pathways
- JAK-STAT Signaling
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