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FGF2 Protein (AA 144-288)

FGF2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Purity is >98 % , as determined by Coomassie stained SDS-PAGE. ICFC Active
Catalog No. ABIN2667407
  • Target See all FGF2 Proteins
    FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 144-288
    Origin
    • 25
    • 10
    • 8
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 40
    • 9
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Application
    Intracellular Flow Cytometry (ICFC)
    Purity
    Purity is >98 % , as determined by Coomassie stained SDS-PAGE.
    Sterility
    0.22 μm filtered
    Endotoxin Level

    Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.

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    Discover our top product FGF2 Protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Biological activity: ED50 = 1 - 4 ng/ml, corresponding to a specific activity of 1 - 0.25 x 106 units/mg, as determined by the dose dependent stimulation of NIH/ 3T3 cell proliferation. The bioactivity is equivalent to competitor reported values.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
    Buffer
    0.22 μm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2, 1 mM DTT.
    Preservative
    Dithiothreitol (DTT)
    Precaution of Use
    This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze/thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
  • Target
    FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
    Alternative Name
    FGF-Basic (FGF2 Products)
    Synonyms
    BFGF Protein, FGF-2 Protein, FGFB Protein, HBGF-2 Protein, Fgf-2 Protein, Fgfb Protein, bFGF Protein, fibroblast growth factor 2 Protein, fibroblast growth factor 2 (basic) Protein, FGF2 Protein, Fgf2 Protein, fgf2 Protein
    Background
    FGFb is a member of the fibroblast growth factor (FGF) family which includes 23 members. FGFb is expressed in almost all tissues and play important roles in a variety of normal and pathological processes, including development, wound healing and neoplastic transformation. FGFb is mitogenic for many cell types, both epithelial and mesenchymal. FGFb shows potent angiogenic activity and has been implicated in tumor angiogenesis (2). In prostate, bladder, and renal cancers, FGFb regulates the induction of metalloproteinases (MMP) that degrade extracellular matrix proteins, thus facilitating tumor metastasis (3). FGFb binds to a family of four distinct, high affinity tyrosine kinase receptors, designated FGFR-1 to -4 (4). In addition, FGFb binds to the ECM, and heparan sulfate (HS) is an essential and dynamic regulator of fibroblast growth factor (FGF) signaling. Two fundamentally different crystallographic models have been proposed to explain, at the molecular level, how HS/heparin enables FGF and FGF receptor (FGFR) to assemble into a functional dimer on the cell surface (5), although there is controversy regarding the exact manner by which this occurs.
    Molecular Weight
    The 145 amino acid recombinant protein has a predicted molecular mass of 16,310 Da. The DTT-reduced and non-reduced protein migrate at approximately 18kDa by SDS-PAGE. This protein may or may not contain an N-terminal methionine.
    Pathways
    RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins
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