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GDNF Protein (AA 78-211, N-Term)

GDNF Origin: Human Host: Escherichia coli (E. coli) Recombinant >98 % , as determined by Coomassie stained SDS-PAGE. FACS Active
Catalog No. ABIN2667420
  • Target See all GDNF Proteins
    GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    N-Term, AA 78-211
    Origin
    • 13
    • 7
    • 3
    • 1
    Human
    Source
    • 13
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Application
    Flow Cytometry (FACS)
    Purity
    >98 % , as determined by Coomassie stained SDS-PAGE.
    Endotoxin Level

    Less than 0.1 ng per μg of protein.

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    Discover our top product GDNF Protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Biological activity: ED50 ≤0.1 ng/ml, corresponding to a specific activity of ≥ 1.0 x 107 units/mg, as measured by its ability to stimulate proliferation of rat C6 cells in a dose dependent manner.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store in working aliquots at -20 °C to -80 °C.
    Buffer
    Lyophilized, carrier-free.
    Handling Advice
    Avoid repeated freeze/thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Unopened vial can be stored at -20°C or -70°C.
  • Target
    GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
    Alternative Name
    GDNF (GDNF Products)
    Synonyms
    gdnf Protein, GDNF Protein, AI385739 Protein, gdnfa Protein, hfb1-gdnf Protein, ATF1 Protein, ATF2 Protein, HFB1-GDNF Protein, HSCR3 Protein, glial cell derived neurotrophic factor Protein, glial cell derived neurotrophic factor a Protein, glial cell line derived neurotrophic factor Protein, glial cell derived neurotrophic factor L homeolog Protein, Gdnf Protein, gdnfa Protein, GDNF Protein, gdnf.L Protein
    Background
    Glial cell line-derived neurotrophic factor (GDNF) is a member of the TGF-β superfamily and was first identified as a growth factor supporting the survival of embryonic midbrain neurons. GDNF has been shown to promote the development, differentiation, and survival of dopaminergic neurons by signaling through GDNF family receptor α-1 (GFRα1) and the coreceptor Ret receptor tyrosine kinase. Dysregulated expression of GDNF has been reported to be associated with several mental diseases, including depression and schizophrenia. GDNF is considered as a promising therapeutic agent for the treatment of Parkinson's disease, since GNDF can protect dopaminergic neurons from neurotoxin and promotes neuron regeneration after injury. In addition to the functions in the nervous system, GDNF is also required for the differentiation of spermatogonia and development of kidney. Upregulated expression of GNDF was observed in various cancer cell lines and correlates with malignant cancer progression, suggesting a role of GDNF in carcinogenesis.
    Molecular Weight
    The 135 amino acid recombinant protein has a predicted molecular mass of approximately 15.0 kDa. Recombinant human GDNF forms a a disulfide-linked homodimer. The predicted N-terminal amino acid is Met.
    Pathways
    RTK Signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration
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