IL-15 Protein

Catalog No. ABIN2667463

Product Details

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purity: >98 % , as determined by Coomassie stained SDS-PAGE.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.,Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Prior to use, reconstitute the lyophilized powder with 0.2 mLof PBS containing a carrier protein (e.g., 1 % BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70 °C for up to one month.

Buffer: Lyophilized in sterile-filtered PBS, pH 7.2, containing 1 % BSA.

Handling Advice: Use a manual defrost freezer and avoid repeated freeze thaw cycles.

Storage: 4 °C

Storage Comment: Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt.

Expiry Date: 12 months

Target Details

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Alternative Name: IL-15 (IL15 Products)

Synonyms: IL15 Protein, AI503618 Protein, IL-15 Protein, interleukin 15 Protein, IL15 Protein, Il15 Protein

Background: Interleukin-15 (IL-15) was discovered in supernatant from simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2 (1). IL-15 is a regulatory cytokine that is produced by dendritic cells, epithelial cells, human stromal cell line (IMTLH), fibroblasts, and monocytes (2). It plays an important role in immune response and shares many functions with IL-2, for example, it stimulates the proliferation of activated T cells (1, 2), NK cells (3) and B cells, and it induces immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand (4). In addition, IL-15 promotes the development of dendritic cells (5), activates human neutrophils (6, 7) and induces the production of proinflammatory cytokines from macrophages (8). IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system. IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL-15Rβ, and IL-15Rγc. IL-15 shares with IL-2 the receptor chains β and γc. IL-15 is normally not secreted in soluble form but is held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 then is presented in trans to T cells and NK cells and is recognized by the γc receptor on these cells, such recognition maintains cell survival and intermittent proliferation (9).

Molecular Weight: The 114 amino acid recombinant protein has a predicted molecular mass of 12.8 kDa. The DTT-reduced protein migrates at approximately 9 kDa and the non-reduced protein migrates at approximately 7 kDa by SDS-PAGE. The N-terminal amino acid is Asn.

Pathways: JAK-STAT Signaling, Glycosaminoglycan Metabolic Process