IL16 Protein (AA 10-130)

Catalog No. ABIN2667464

Product Details

Target: IL16 (Interleukin 16 (IL16))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 10-130

Origin: Human

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

Endotoxin Level:

Less than 0.1 ng per μg of protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Determined by its ability to chemoattract human peripheral blood lymphocytes using a concentration range of 50.0 - 1000.0 ng/ml.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -20°C or -70°C.

Target Details

Target: IL16 (Interleukin 16 (IL16))

Alternative Name: IL-16 (IL16 Products)

Synonyms: IL16 Protein, LCF Protein, NIL16 Protein, PRIL16 Protein, prIL-16 Protein, mKIAA4048 Protein, IL-16 Protein, interleukin 16 Protein, IL16 Protein, Il16 Protein

Background: IL-16 is a proinflammatory cytokine synthesized as a 631-amino acid precursor protein (pro-IL-16). The pro-IL-16 is expressed at high level in T cells. After cell activation via the T cell rceptor, the C-terminal cytokine domain (121 amino acids) can be released from the N-terminal prodomain by caspase-3 and become biological active. Self-aggregation into homotetramer is required for its biological activities. IL-16 is a chemoattractant for CD4+ T cells, monocytes, and eosinophils. IL-16 also induces the expression of CD25 and activates CD4+ T cells synergically with IL-2 or IL-15. IL-16 can also induce IL-1, IL-6 and TNF-α production in monocytes. It has been shown tha IL-16 can inhibit HIV-1 replication in vitro by repressing the transcription of the HIV-1 long terminal repeat. The pro-IL-16 has been identified as a T lymphocyte cell cycle growth suppressor. In primary T cells, nuclear presence of pro-IL-16 results in a resting state. Pro-IL-16 can function as a scadffold protein which can interact with GA-binding protein β, HDAC3, and heat shock protein 70.

Molecular Weight: The 121 amino acid recombinant protein has a predicted molecular mass of approximately 12.4 kDa. The predicted N-terminal amino acid is Ser.