IL1RN Protein (AA 26-177)

Catalog No. ABIN2667496

Product Details

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 26-177

Origin: Human

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

Endotoxin Level:

Less than 0.1 ng per μg of protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 is 20 - 40 ng/ml in the presence of 50 pg/ml of IL-1α, corresponding to a specific activity of 2.5 - 5.0 x 105 units/mg as determined by its ability to inhibit the IL-1α stimulation of murine D10S cell.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -20°C or -70°C.

Target Details

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Alternative Name: IL-1RA (IL1RN Products)

Synonyms: DIRA Protein, ICIL-1RA Protein, IL-1RN Protein, IL-1ra Protein, IL-1ra3 Protein, IL1F3 Protein, IL1RA Protein, IRAP Protein, MVCD4 Protein, IRAP1 Protein, IL1RN Protein, IL-1RA Protein, F630041P17Rik Protein, interleukin 1 receptor antagonist Protein, interleukin 1 receptor antagonist-like Protein, IL1RN Protein, Il1rn Protein, IL1RNL Protein

Background: Interleukin-1 receptor antagonist (IL-1RA or IL-RN) is a member of IL-1 family cytokine and it is a naturally occurring inflammatory inhibitor protein. It competes with IL-1α and IL-1β for binding to type I and type II receptors. It can modulate a variety of interleukin 1 related immune and inflammatory responses. IL-1RA has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo model. Recombinant IL-1RA (anakinra) has been used as to treat rheumatoid arthritis. A polymorphism of IL-1RN gene is reported to be associated with increased risk of osteoporotic fractures, gastric cancer, bladder cancer, and other cancer types.

Molecular Weight: The 153 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 17.2 kDa. The predicted N-terminal amino acid is Met.

Pathways: NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints