Neurotrophin 3 Protein (NTF3) (AA 139-257, N-Term)

Catalog No. ABIN2667561

Product Details

Target: Neurotrophin 3 (NTF3)

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 139-257, N-Term

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >98 % , as determined by SDS-PAGE and HPLC analysis.

Endotoxin Level:

Less than 0.1 ng per μg of protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found in the range of 20-50 ng/ml, corresponding to a specific activity of 2-5 x 104 units/mg.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -20°C or -70°C.

Target Details

Target: Neurotrophin 3 (NTF3)

Alternative Name: NT-3 (NTF3 Products)

Synonyms: HDNF Protein, NGF-2 Protein, NGF2 Protein, NT3 Protein, NT-3 Protein, wu:fl98d04 Protein, hdnf Protein, ngf-2 Protein, ngf2 Protein, nt-3 Protein, nt3 Protein, NTF3 Protein, AI316846 Protein, AI835689 Protein, Nt3 Protein, Ntf-3 Protein, neurotrophin 3 Protein, neurotrophin 3 S homeolog Protein, neurotrophin 3 L homeolog Protein, NTF3 Protein, ntf3 Protein, ntf3.S Protein, Ntf3 Protein, ntf3.L Protein

Background: Neurotrophins are growth factors that modulate growth, differentiation, and survival of neurons. Structure related proteins include neuron growth factor (NGF), brain derived neurogenic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4/5. They are highly homologous and share a similar structure of a tertiary folded, cysteine rich "knot". Neurotrophins are critical to the development of the central and peripheral nervous system and they play important roles in injury induced neuron regeneration. NT-3 is encoded by the the NTF3 gene and shares high similarity among species including human, pig, rat, and mouse. It is the only neurotrophin that affects the development of the enteric nervous system. NT-3 can act on neuroblast/progenitor cells directly or synergistically with other neurotrophins. It can regulate growth and maturation of neurons in both peripheral and central nervous systems. NT-3 elicits its activity mainly through activation of p75, tropomyosin-related kinase receptors (Trk), TrkB, and TrkC. NT-3 is the only ligand for TrkC and studies indicate that neuron generation in myenteric and submucosal plexuses is blocked in mice with deficiency in NT-3/TrkC signaling. The expression of NT-3 is regulated by bone morphogenetic protein 2 (BMP-2) and bone morphogenetic protein 4 (BMP-4). Although the NT-3-TrkC signal transduction is known to lead cellular apoptosis in the absence of ligand, overexpression of NT-3 conversely disrupts the tumor-preventive control and results in tumor generation. Dysregulation of NT-3 is known to be related with neuroblastoma, Alzhiemer's, Huntington's, and Parkinson's Disease. Due to the broad cell poplulations affected by NT-3, the control of NT-3-TrkC pathway has become a target for therapeutical approach for neurogenic diseases.

Molecular Weight: NT-3 recombinant protein is a noncovalent linked homodimer. The 120 amino acid recombinant protein has a predicted molecular mass of approximately 13.6 kDa as monomer, 26.5 kDa as dimer. The predicted N-terminal amino acid is Met.

Pathways: RTK Signaling, Neurotrophin Signaling Pathway