FAK Protein (AA 2-1052, GST-His-Tag) (GST-His-Tag)
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- Target See all FAK (PTK2) Proteins
- FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 2-1052, GST-His-Tag
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Origin
- Human
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Source
- Baculovirus
- Purification tag / Conjugate
- This FAK protein is labelled with GST-His-Tag.
- Application
- Western Blotting (WB), Kinase Activity Assay (KAA)
- Characteristics
- Recombinant human FAK protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids A2-H1052, accession number L13616 and N-terminally fused to GST-HIS6TagTagTag-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
- Purification
- Purified by GSH-agarose affinity purification.
- Top Product
- Discover our top product PTK2 Protein
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PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 1-680) protein (GST tag)
PTK2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA, KAA
PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 1-527) (Active) protein (GST tag)PTK2 Origin: Human Host: Insect cells (Sf21) Recombinant SDS, Func Active
PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 2-1052) (Active) protein (GST tag)PTK2 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 845-1052) protein (His tag)PTK2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 355-490) protein (GST tag)PTK2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 2-1052) protein (His tag)Crystallography grade PTK2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 2-1090) protein (His tag)Crystallography grade PTK2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
PTK2 Protein tyrosine Kinase 2 (PTK2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PTK2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Recombinant FAK is suitable for kinase assays and Western blot. The molecular weight of the protein is ~145.394 kDa. The activity of the protein is ~ 1.5 pmol/μg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 μM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 μg/50 μL PEG20.000, Substrate: PolyEY 0.5 μg/50 μL, Recombinant FAK: 200 ng/50 μL. Kinase activity may vary depending on the substrate and reaction conditions used.
- Restrictions
- For Research Use only
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- Concentration
- 0.135 μg/μL
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- Target
- FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
- Alternative Name
- FAK (PTK2 Products)
- Pathways
- Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
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