DNMT1 Protein (AA 2-1632) (GST tag)
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- Target See all DNMT1 Proteins
- DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 2-1632
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Origin
- Human
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Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- This DNMT1 protein is labelled with GST tag.
- Application
- Enzyme Activity Assay (EAA)
- Characteristics
- Recombinant human DNMT1 was expressed in Sf9 cells via a baculovirus expression system as amino acids 2-1632 (accession number NM_001130823) with an N-terminal GST tag. The molecular weight of the protein is 211 kDa.
- Top Product
- Discover our top product DNMT1 Protein
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DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (Active) protein
DNMT1 Origin: Mouse Host: Insect Cells Recombinant > 95 % protein determined by SDS-PAGE (CBB staining) Active
DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) protein (His tag)DNMT1 Origin: Rat Host: Escherichia coli (E. coli) Recombinant >80 % as determined by reducing SDS-PAGE.
DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) protein (His tag)DNMT1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >80 % as determined by reducing SDS-PAGE.
DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 1-110) protein (GST tag)DNMT1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 1-1616) protein (Strep Tag)Crystallography grade DNMT1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 1-1620) protein (Strep Tag)Crystallography grade DNMT1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)DNMT1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
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- Application Notes
- Recombinant DNMT1 is suitable for use in DNA methylation assays. This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
- Restrictions
- For Research Use only
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- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))
- Alternative Name
- DNMT1 (DNMT1 Products)
- Synonyms
- cb1075 Protein, cb983 Protein, dmnt1 Protein, fb05h01 Protein, mta Protein, wu:fb05h01 Protein, ADCADN Protein, AIM Protein, CXXC9 Protein, DNMT Protein, HSN1E Protein, MCMT Protein, DDM2 Protein, DECREASED DNA METHYLATION 2 Protein, DMT01 Protein, DMT1 Protein, DNA METHYLTRANSFERASE Protein, DNA METHYLTRANSFERASE 01 Protein, DNA METHYLTRANSFERASE 1 Protein, MET2 Protein, METHYLTRANSFERASE 2 Protein, METHYLTRANSFERASE I Protein, METI Protein, methyltransferase 1 Protein, Cxxc9 Protein, Dnmt Protein, Dnmt1o Protein, MTase Protein, Met-1 Protein, Met1 Protein, MommeD2 Protein, m.MmuI Protein, DNA methyltransferase 1 Protein, DNA (cytosine-5-)-methyltransferase 1 Protein, methyltransferase 1 Protein, DNA methyltransferase (cytosine-5) 1 Protein, DNMT1 Protein, dnmt1 Protein, Dnmt1 Protein, MET1 Protein
- Background
- DNMT1 - DNA methyltransferase 1 (or DNMT 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.
- Molecular Weight
- The molecular weight of the protein is 211 kDa.
- Pathways
- SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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