HDAC4 Protein (AA 645-1057, Catalytic Domain)

Catalog No. ABIN2669629

Product Details

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Catalytic Domain, AA 645-1057

Origin: Human

Source: Escherichia coli (E. coli)

Application: Enzyme Activity Assay (EAA)

Characteristics: HDAC4 is a Class II histone deacetylase with broad substrate specificity. The catalytic domain corresponding to amino acids 645 - 1057 of HDAC4 (accession number NP_006028.2) was expressed in E. coli with an apparent molecular weight of 45 kDa. An epitope tag present during expression was removed proteolytically after purification.

Application Details

Application Notes: Recombinant HDAC4 is suitable for use in histone deacetylase (HDAC) assays. A good starting point is 2 to 5 ng of enzyme per assay.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Target Details

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Alternative Name: HDAC4 (HDAC4 Products)

Synonyms: CG1770 Protein, DHDAC4 Protein, Dmel\\CG1770 Protein, GC1770 Protein, HDAC Protein, HDAC4a Protein, dHDAC4 Protein, dmHDA405 Protein, hdac4 Protein, dana_GLEANR_19966 Protein, DanaGF18710 Protein, GF18710 Protein, HDAC4 Protein, AHO3 Protein, BDMR Protein, HA6116 Protein, HD4 Protein, HDAC-4 Protein, HDAC-A Protein, HDACA Protein, 4932408F19Rik Protein, AI047285 Protein, wu:fa96d08 Protein, wu:fc56f08 Protein, zgc:152701 Protein, histone deacetylase 4 Protein, hypothetical protein Protein, Histone deacetylase 4 Protein, GF18710 gene product from transcript GF18710-RC Protein, PTRG_03699 Protein, PGTG_15512 Protein, HDAC4 Protein, Dana\GF18710 Protein, Hdac4 Protein, hdac4 Protein

Background: HDAC4 (Histone Deacetylase 4) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and 10), class III (SIRT1-7) and class IV (HDAC11). Unlike other deacetylases, HDAC4 shuttles between the nucleus and cytoplasm and serves as a nuclear co-repressor that regulates bone and muscle development. HDAC4 interacts with the myocyte enhancer factors Mef2a, Mef2c and Mef2d. It also forms part of a multi-protein complex with RbAp48 and HDAC3. HDAC4 is ubiquitous.

Molecular Weight: 45 kDa

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process