BRD2 Protein (AA 344-455) (His tag,DYKDDDDK Tag)

Catalog No. ABIN2669643

Product Details

Target: BRD2 (Bromodomain Containing 2 (BRD2))

Protein Type: Recombinant

Protein Characteristics: AA 344-455

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This BRD2 protein is labelled with His tag,DYKDDDDK Tag.

Application: Binding Studies (Bind), Screening Assay (ScA)

Characteristics: The peptide corresponding to amino acids 344 - 455 that contains the bromodomain sequences of BRD2 (accession number NM_005104.3) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 19.4 kDa. It shows binding specificity for acetylated H4K5/K8, H4K12, H4K8/K12, H4K12/K16, H4K12/K16/K20 and H4K5/K8/K12/K16.

Application Details

Application Notes: Recombinant BRD2 (344-455) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Target Details

Target: BRD2 (Bromodomain Containing 2 (BRD2))

Alternative Name: BRD2 (BRD2 Products)

Synonyms: D6S113E Protein, FSH Protein, FSRG1 Protein, NAT Protein, RING3 Protein, RNF3 Protein, AW228947 Protein, D17H6S113E Protein, Frg-1 Protein, Fsrg-1 Protein, Fsrg1 Protein, Nat Protein, Ring3 Protein, Rnf3 Protein, mKIAA4005 Protein, brd2-a Protein, fsh Protein, fsrg1 Protein, nat Protein, ring3 Protein, rnf3 Protein, brd2 Protein, fe47f02 Protein, fi85h05 Protein, wu:fe47f02 Protein, wu:fi85h05 Protein, Ring3 protein Protein, bromodomain containing 2 Protein, bromodomain containing 2 S homeolog Protein, bromodomain containing 2a Protein, CHLREDRAFT_190116 Protein, CHLREDRAFT_150233 Protein, CHLREDRAFT_182576 Protein, CHLREDRAFT_188250 Protein, BRD2 Protein, Brd2 Protein, brd2.S Protein, brd2a Protein

Background: Bromodomain-containing protein 2 (BRD2), also known as RING3, belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD proteins are related to the female sterile homeotic protein gene in Drosophila, a gene required maternally for proper expression of other homeotic genes, such as Ubx, which is involved in pattern formation. BRD2 causes elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. BRD2 has been shown to interact with E2F1 and with histone H4 acetylated at Lys12 via its two bromodomains. BRD2 may play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses.

Molecular Weight: 19.4 kDa

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection