BRD3 Protein (AA 24-144) (His tag,DYKDDDDK Tag)

Catalog No. ABIN2669644

Product Details

Target: BRD3 (Bromodomain Containing 3 (BRD3))

Protein Type: Recombinant

Protein Characteristics: AA 24-144

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This BRD3 protein is labelled with His tag,DYKDDDDK Tag.

Characteristics: The peptide corresponding to amino acids 24 - 144 that contains the bromodomain sequences of BRD3 (accession number NM_007371.3) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 20.5 kDa. It shows binding specificity for acetylated H3K18, H4K12, H4K20 and H4K12/K16/K20.

Application Details

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Target Details

Target: BRD3 (Bromodomain Containing 3 (BRD3))

Alternative Name: BRD3 (BRD3 Products)

Synonyms: ORFX Protein, RING3L Protein, 2410084F24Rik Protein, AW060456 Protein, Fsrg2 Protein, RINGL3 Protein, brd3 Protein, fi36b10 Protein, wu:fa13h06 Protein, wu:fc01d09 Protein, wu:fc74a08 Protein, wu:fi04h10 Protein, wu:fi36b10 Protein, BRD3 Protein, ring3l Protein, fa28f05 Protein, wu:fa28f05 Protein, wu:fc56a11 Protein, wu:fj66d12 Protein, zgc:77289 Protein, bromodomain containing 3 Protein, bromodomain containing 3a Protein, bromodomain containing 3 S homeolog Protein, bromodomain-containing protein 3 Protein, bromodomain containing 3b Protein, BRD3 Protein, Brd3 Protein, brd3a Protein, brd3.S Protein, LOC100410652 Protein, LOC100354641 Protein, brd3b Protein

Background: Bromodomain-containing protein 3 (BRD3), also known as RING3L, belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD3 binds and regulates GATA1 in an acetylation-dependent manner. GATA1 is a key regulator of gene expression for erythroid and megakaryocyte-specific genes, and mutations in GATA1 have been associated with congenital anemias and megakaryoblastic leukemias. Interestingly, tight interaction of BRD3 with GATA1 requires multiple acetylation modifications, and structural data showed that two adjacent acetylation sites in GATA1 interact with a single bromodomain. BRD3 protein expression is induced in activated lymphocytes. Additionally, it is highly expressed in undifferentiated ES cells and expression is observed to drop upon endothelial differentiation. Altered expression levels of BRD3 have been observed in certain cancers, such as nasopharyngeal carcinomas and bladder cancer. BRD3 also interacts with LANA-1, the Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen 1, which is required for the replication of episomal viral genomes.

Molecular Weight: 20.5 kDa

Pathways: Chromatin Binding