PRMT6 Protein (DYKDDDDK Tag)
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- Target See all PRMT6 Proteins
- PRMT6 (Protein Arginine Methyltransferase 6 (PRMT6))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus
- Purification tag / Conjugate
- This PRMT6 protein is labelled with DYKDDDDK Tag.
- Application
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Characteristics
- Recombinant PRMT6 (accession number NP_060607.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 43.7 kDa.
- Top Product
- Discover our top product PRMT6 Protein
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- Application Notes
- Recombinant PRMT6 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 60 ng/μl Histone H3 purified from E. coli (rH3), 30 ng/μl Recombinant PRMT6 protein at 2 hours at room temperature. Activity was detected by fluorography.
- Restrictions
- For Research Use only
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- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- PRMT6 (Protein Arginine Methyltransferase 6 (PRMT6))
- Alternative Name
- PRMT6 (PRMT6 Products)
- Synonyms
- CG9927 Protein, DART6 Protein, Dmel\\CG9927 Protein, PRMT6 Protein, HRMT1L6 Protein, ARABIDOPSIS THALIANA PROTEIN ARGININE METHYLTRANSFERASE 6 Protein, ATPRMT6 Protein, protein arginine methyltransferase 6 Protein, DKFZp459B1431 Protein, Hrmt1l6 Protein, hrmt1l6 Protein, hrmt6 Protein, im:6908706 Protein, AW124876 Protein, BB233495 Protein, Arginine methyltransferase 6 Protein, protein arginine methyltransferase 6 Protein, protein arginine methyltransferase 6 S homeolog Protein, arginine N-methyltransferase, type I Protein, protein arginine N-methyltransferase 6 Protein, Art6 Protein, PRMT6 Protein, prmt6.S Protein, prmt6 Protein, Prmt6 Protein
- Background
- PRMT6 (Protein Arginine Methyltransferase 6, HRMT1L6, ANM6) is a type I arginine methyltransferase. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT6 preferentially methylates arginine residues present in GAR (glycine and arginine-rich) domains. PRMT6 asymmetrically dimethylates histone H3 at Arg2, which is mutually exclusive to methylation at lysine 4 (associated with transcriptional activation), and thus PRMT6 serves as a transcriptional repressor in this context. It can also symmetrically dimethylate H2A and H4 at Arg3 and serves as a coactivator of transcription in concert with nuclear receptors.
- Molecular Weight
- 43.7 kDa
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