SMYD3 Protein (DYKDDDDK Tag)
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- Target See all SMYD3 Proteins
- SMYD3 (SET and MYND Domain Containing 3 (SMYD3))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus
- Purification tag / Conjugate
- This SMYD3 protein is labelled with DYKDDDDK Tag.
- Application
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Characteristics
- Recombinant Smyd3 (accession number NP_001161212.1) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 50.7 kDa.
- Top Product
- Discover our top product SMYD3 Protein
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SET and MYND Domain Containing 3 (SMYD3) (AA 1-369) protein (GST tag)
SMYD3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
SET and MYND Domain Containing 3 (SMYD3) (AA 240-349) protein (GST tag)SMYD3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
SET and MYND Domain Containing 3 (SMYD3) (AA 1-428) protein (Strep Tag)Crystallography grade SMYD3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
SET and MYND Domain Containing 3 (SMYD3) (AA 1-428) protein (Strep Tag)Crystallography grade SMYD3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
SET and MYND Domain Containing 3 (SMYD3) protein (Myc-DYKDDDDK Tag)SMYD3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Recombinant Smyd3 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: Specifically mediates the methylation of 'Lys-4' and 'Lys-5' of histone H3 and may methylate non-histone substrates. HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 60 ng/μl non-histone peptide, 30 ng/μl Recombinant Smyd3 protein at 2 hours at room temperature. Activity was detected by fluorography.
- Restrictions
- For Research Use only
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- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- SMYD3 (SET and MYND Domain Containing 3 (SMYD3))
- Alternative Name
- SMYD3 (SMYD3 Products)
- Synonyms
- KMT3E Protein, ZMYND1 Protein, ZNFN3A1 Protein, bA74P14.1 Protein, 2410008A19Rik Protein, Zmynd1 Protein, SET and MYND domain containing 3 Protein, SMYD3 Protein, Smyd3 Protein
- Background
- SET and MYND domain-containing protein 3 (Smyd3) is a member of the Smyd family of histone methyltransferases that specifically methylates lysine 4 of histone H3 (H3K4), inducing di- and tri-methylation, but not monomethylation. Smyd3 plays an important role in transcriptional activation as a member of an RNA polymerase complex. It has also been shown to bind DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. Smyd3 expression has been shown to be upregulated in certain cancers.
- Molecular Weight
- 50.7 kDa
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