TAF1 Protein (AA 1522-1656) (His tag,DYKDDDDK Tag)

Catalog No. ABIN2669687

Product Details

Target: TAF1 (TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1))

Protein Type: Recombinant

Protein Characteristics: AA 1522-1656

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TAF1 protein is labelled with His tag,DYKDDDDK Tag.

Application: Binding Studies (Bind), Screening Assay (ScA)

Characteristics: The peptide corresponding to amino acids 1522-1656 that contains the bromodomain sequences of TAF1 (accession number NP_004597.2) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 21.75 kDa. It shows binding specificity for acetylated H3K14.

Application Details

Application Notes: Recombinant TAF1 (1522-1656) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Target Details

Target: TAF1 (TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1))

Alternative Name: TAF1 (TAF1 Products)

Synonyms: BG:DS00004.13 Protein, CG17603 Protein, Dmel\\CG17603 Protein, EfW1 Protein, SR3-5 Protein, TAF Protein, TAF1 Protein, TAF200 Protein, TAF230 Protein, TAF250 Protein, TAF250/230 Protein, TAFII-250 Protein, TAFII250 Protein, TAF[II]250 Protein, TAF[[II]] Protein, TAF[[II]]230 Protein, TAF[[II]]250 Protein, TAF[[II]]250/230 Protein, TFIID Protein, TFIID TAF250 Protein, Taf1p Protein, Taf200 Protein, Taf230 Protein, Taf250 Protein, Taf[[II]]250 Protein, cel Protein, cell Protein, d230 Protein, dTAF230 Protein, dTAF250 Protein, dTAFII250 Protein, dTAF[[II]]230 Protein, dTAF[[II]]250 Protein, dmTAF1 Protein, dmTAF[[II]]230 Protein, l(3)84Ab Protein, p230 Protein, 250kDa Protein, AU015687 Protein, B430306D02Rik Protein, Ccg-1 Protein, Ccg1 Protein, KAT4 Protein, N-TAF1 Protein, Taf2a Protein, RGD1562050 Protein, BA2R Protein, CCG1 Protein, CCGS Protein, DYT3 Protein, DYT3/TAF1 Protein, NSCL2 Protein, OF Protein, P250 Protein, TAF2A Protein, XDP Protein, TAF(II)250 Protein, p250 Protein, TBP-associated factor 1 Protein, TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa Protein, transcription initiation factor TFIID subunit 1 Protein, transcription initiation factor TFIID, subunit TAF1 Protein, TATA-box binding protein associated factor 1 Protein, Taf1 Protein, TAF1 Protein, LOC5567420 Protein, CpipJ_CPIJ003658 Protein, TAF11.1 Protein, HAF1501 Protein, HAF1502 Protein, LOC100457071 Protein

Background: TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex. TAF1 contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. TAF1 also contains bromodomains for interaction with other proteins. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TAF1 has been shown to phosphorylate threonine 55 of p53 which leads to MDM2-mediated degradation of p53. TAF1 also phosphorylates GTF2A1 and GTF2F1 on serine residues. In addition, TAF1 possesses DNA-binding activity and is essential for progression of the G1 phase of the cell cycle.

Molecular Weight: 21.75 kDa