SETDB1 Protein (DYKDDDDK Tag)
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- Target See all SETDB1 Proteins
- SETDB1 (SET Domain, Bifurcated 1 (SETDB1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus
- Purification tag / Conjugate
- This SETDB1 protein is labelled with DYKDDDDK Tag.
- Application
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Characteristics
- Recombinant SETDB1 (accession number NP_001138887.1) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 145 kDa.
- Top Product
- Discover our top product SETDB1 Protein
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SET Domain, Bifurcated 1 (SETDB1) (AA 2-100) protein (GST tag)
SETDB1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
SET Domain, Bifurcated 1 (SETDB1) (AA 247-348) protein (GST tag)SETDB1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
SET Domain, Bifurcated 1 (SETDB1) (AA 1-1291) protein (Strep Tag)Crystallography grade SETDB1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
SET Domain, Bifurcated 1 (SETDB1) (AA 1-1307) protein (Strep Tag)Crystallography grade SETDB1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
SET Domain, Bifurcated 1 (SETDB1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)SETDB1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
SET Domain, Bifurcated 1 (SETDB1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SETDB1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Recombinant SETDB1 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me3 methyltransferase. HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM, 30 ng/μl Calf Thymus Histone Octamer, 30 ng/μl Recombinant SETDB1 protein at 2 hours at room temperature. Activity was detected by fluorography.
- Restrictions
- For Research Use only
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- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- SETDB1 (SET Domain, Bifurcated 1 (SETDB1))
- Alternative Name
- SETDB1 (SETDB1 Products)
- Synonyms
- ESET Protein, H3-K9-HMTase4 Protein, KG1T Protein, KMT1E Protein, TDRD21 Protein, AU022152 Protein, mKIAA0067 Protein, SETDB1 Protein, NV10069 Protein, fc07d03 Protein, si:ch211-81a5.6 Protein, wu:fc07d03 Protein, im:7143091 Protein, wu:fa04a12 Protein, wu:fj80h02 Protein, zgc:152899 Protein, SET domain bifurcated 1 Protein, SET domain, bifurcated 1 Protein, myeloid/lymphoid or mixed-lineage leukemia; translocated to, 11 Protein, histone-lysine N-methyltransferase SETDB1 Protein, SET domain, bifurcated 1a Protein, SET domain bifurcated 1 L homeolog Protein, SET domain, bifurcated 1b Protein, SETDB1 Protein, Setdb1 Protein, MLLT11 Protein, LOC100120014 Protein, LOC100461671 Protein, setdb1a Protein, setdb1.L Protein, setdb1b Protein
- Background
- SETDB1 (SET Domain, Bifurcated 1) is a histone methyltransferase that methylates histone H3 at lysine 9. H3K9 methylation is associated with transcriptional repression and gene silencing through recruitment of HP1 proteins to methylated histones. H3K9 trimethylation is coordinated with DNA methylation, most likely through formation of a complex with MBD1 and ATF7IP that leads to transcriptional repression. SETDB1 activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28, a factor recruited by KRAB zinc-finger proteins. Aberrant SETDB1 function is associated with the pathogenesis of Huntington Disease.
- Molecular Weight
- 145 kDa
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