JMJD2D Protein (DYKDDDDK Tag)
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- Target See all JMJD2D (KDM4D) Proteins
- JMJD2D (KDM4D) (Lysine (K)-Specific Demethylase 4D (KDM4D))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus
- Purification tag / Conjugate
- This JMJD2D protein is labelled with DYKDDDDK Tag.
- Application
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Characteristics
- Recombinant JMJD2D / KDM4D (accession number NP_060509.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 63.2 kDa.
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Lysine (K)-Specific Demethylase 4D (KDM4D) (AA 1-523) protein (GST tag)
KDM4D Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Lysine (K)-Specific Demethylase 4D (KDM4D) (AA 1-523) protein (His tag)Crystallography grade KDM4D Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Lysine (K)-Specific Demethylase 4D (KDM4D) (AA 1-510) protein (His tag)Crystallography grade KDM4D Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
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- Application Notes
- Recombinant JMJD2D / KDM4D is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me3 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 100 nM Recombinant JMJD2D / KDM4D protein, and 3.3 μM H3K9me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
- Restrictions
- For Research Use only
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- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- JMJD2D (KDM4D) (Lysine (K)-Specific Demethylase 4D (KDM4D))
- Alternative Name
- JMJD2D / KDM4D (KDM4D Products)
- Background
- KDM4D (lysine (K)-specific demethylase 4D), also known as JMJD2D (Jumonji Domain Containing 2D) is a protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 9 residues of histone H3, while it has no activity on monomethylated H3K9 residues.
- Molecular Weight
- 63.2 kDa
- Pathways
- Warburg Effect
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