KDM1B Protein (full length) (GST tag)
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- Target See all KDM1B Proteins
- KDM1B (Lysine (K)-Specific Demethylase 1B (KDM1B))
- Protein Type
- Recombinant
- Protein Characteristics
- full length
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Origin
- Human
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Source
- Baculovirus
- Purification tag / Conjugate
- This KDM1B protein is labelled with GST tag.
- Application
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Characteristics
- Full-length Recombinant KDM1B / LSD2 (accession number XM_005248926.1) was expressed in was expressed in Sf9 cells and contains an N-terminal GST tag with an observed molecular weight of 116 kDa.
- Top Product
- Discover our top product KDM1B Protein
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Lysine (K)-Specific Demethylase 1B (KDM1B) (AA 1-822) protein (Strep Tag)
Crystallography grade KDM1B Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Lysine (K)-Specific Demethylase 1B (KDM1B) (AA 1-826) protein (Strep Tag)Crystallography grade KDM1B Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
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- Application Notes
- Recombinant KDM1B / LSD2 protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me2 and H3K4me1 demethylase. DMT Assay Conditions: 50 mM TrisCl, pH 8.0, 0.02 % Triton X-100, 10 nM FAD, 5 nM KDM1B / LSD2, 3.3 μM H3K4me2 peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
- Restrictions
- For Research Use only
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- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- KDM1B (Lysine (K)-Specific Demethylase 1B (KDM1B))
- Alternative Name
- KDM1B / LSD2 (KDM1B Products)
- Synonyms
- AOF1 Protein, C6orf193 Protein, LSD2 Protein, bA204B7.3 Protein, dJ298J15.2 Protein, 4632428N09Rik Protein, AI482520 Protein, Aof1 Protein, lysine demethylase 1B Protein, lysine-specific histone demethylase 1B Protein, lysine (K)-specific demethylase 1B Protein, KDM1B Protein, LOC577549 Protein, kdm1b Protein, Kdm1b Protein
- Background
- KDM1B / LSD2 (Lysine (K)-Specific Demethylase 1B / Lysine-Specific Histone Demethylase 2) is a histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4 (by similarity).
- Molecular Weight
- 116 kDa
- Pathways
- Warburg Effect
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