P-Selectin Protein

Catalog No. ABIN2691046

Product Details

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Origin: Mouse

Source: Synthetic

Application: Immunoprecipitation (IP), Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The Mouse P-selectin - IgG Fusion Protein is composed of the signal sequence, the lectin domain, the EGF-like repeat, and the first two complement-binding domains of mouse P-selectin (CD62P) fused to the Fc region (hinge, CH2 and CH3) of human IgG1. This chimeric protein binds to P-selectin ligand expressed on mouse neutrophils and myeloid cell lines. Furthermore, P-selectin-IgG fusion protein selectively stains Th1 cells, but not Th2 cells.reported in the literature. Detection of P-Selectin ligands on mouse granulocytes (left panel) and mononuclear cells (right panel). BALB/c whole blood was stained with Mouse P-Selectin - IgG Fusion Protein followed by R-PE-conjugated goat anti-human IgG, Fcγ fragment specific (Jackson Immunoresearch, open histogram) or with the second-step alone (filled histogram). Erythrocytes were lysed (BD Pharm Lyse™ lysis buffer, Cat. No. 555899), non-viable leukocytes were excluded by staining with 7-AAD (BD Via-Probe™ cell viability dye, Cat. No. 555815/555816), and granulocytes and mononuclear cells were gated according to their light-scatter profiles. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

Purification: Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

BD Pharmingen™ Purified Mouse P-Selectin - IgG Fusion Protein - Purified - Clone NA - Reactivity Ms

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Avoid multiple freeze-thaws of product. The fusion protein solution should be stored at -20°C until the vial is opened. After opening, undiluted aliquots should be stored at -20°C or below. Thawed aliquots may be stored up to two months at 4°C.

References

Katakai, Mori, Masuda, Shimizu: "Selective accumulation of type 1 effector cells expressing P-selectin ligand and/or alpha(4)beta(7)-integrin at the lesions of autoimmune gastritis." in: International immunology, Vol. 14, Issue 2, pp. 167-75, (2002) (PubMed).

Austrup, Vestweber, Borges, Löhning, Bräuer, Herz, Renz, Hallmann, Scheffold, Radbruch, Hamann: "P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflammed tissues." in: Nature, Vol. 385, Issue 6611, pp. 81-3, (1997) (PubMed).

Lenter, Levinovitz, Isenmann, Vestweber: "Monospecific and common glycoprotein ligands for E- and P-selectin on myeloid cells." in: The Journal of cell biology, Vol. 125, Issue 2, pp. 471-81, (1994) (PubMed).

Hahne, Jäger, Isenmann, Hallmann, Vestweber: "Five tumor necrosis factor-inducible cell adhesion mechanisms on the surface of mouse endothelioma cells mediate the binding of leukocytes." in: The Journal of cell biology, Vol. 121, Issue 3, pp. 655-64, (1993) (PubMed).

Target Details

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Alternative Name: P-Selectin (SELP Products)

Synonyms: PSELECT Protein, CD62 Protein, CD62P Protein, GMP140 Protein, GRMP Protein, LECAM3 Protein, PADGEM Protein, PSEL Protein, GMP-140 Protein, Grmp Protein, selectin P Protein, selectin, platelet Protein, Selp Protein, SELP Protein