EBP Protein (Myc-DYKDDDDK Tag)
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- Target See all EBP Proteins
- EBP (Emopamil Binding Protein (Sterol Isomerase) (EBP))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This EBP protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human D8-D7 sterol isomerase protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product EBP Protein
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Emopamil Binding Protein (Sterol Isomerase) (EBP) (AA 2-230) protein (rho-1D4 tag)
Crystallography grade EBP Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Emopamil Binding Protein (Sterol Isomerase) (EBP) (AA 2-230) protein (rho-1D4 tag)Crystallography grade EBP Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- EBP (Emopamil Binding Protein (Sterol Isomerase) (EBP))
- Alternative Name
- d8-d7 Sterol Isomerase (EBP Products)
- Synonyms
- cdpx2 Protein, cho2 Protein, cpx Protein, cpxd Protein, zgc:91895 Protein, CDPX2 Protein, CHO2 Protein, CPX Protein, CPXD Protein, AI255399 Protein, Pabp Protein, Td Protein, mSI Protein, emopamil binding protein (sterol isomerase) Protein, emopamil binding protein (sterol isomerase) L homeolog Protein, phenylalkylamine Ca2+ antagonist (emopamil) binding protein Protein, Ebp Protein, ebp.L Protein, ebp Protein, EBP Protein
- Background
- The protein encoded by this gene is an integral membrane protein of the endoplasmic reticulum. It is a high affinity binding protein for the antiischemic phenylalkylamine Ca2+ antagonist [3H]emopamil and the photoaffinity label [3H]azidopamil. It is similar to sigma receptors and may be a member of a superfamily of high affinity drug-binding proteins in the endoplasmic reticulum of different tissues. This protein shares structural features with bacterial and eukaryontic drug transporting proteins. It has four putative transmembrane segments and contains two conserved glutamate residues which may be involved in the transport of cationic amphiphilics. Another prominent feature of this protein is its high content of aromatic amino acid residues (>23 % ) in its transmembrane segments. These aromatic amino acid residues have been suggested to be involved in the drug transport by the P-glycoprotein. Mutations in this gene cause Chondrodysplasia punctata 2 (CDPX2 also known as Conradi-Hunermann syndrome).
- Molecular Weight
- 26.2 kDa
- NCBI Accession
- NP_006570
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