CYP7A1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2712786

Product Details

Target: CYP7A1 (Cytochrome P450, Family 7, Subfamily A, Polypeptide 1 (CYP7A1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CYP7A1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human CYP7A1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: CYP7A1 (Cytochrome P450, Family 7, Subfamily A, Polypeptide 1 (CYP7A1))

Alternative Name: Cyp7a1 (CYP7A1 Products)

Synonyms: CP7A Protein, CYP7 Protein, CYPVII Protein, CHAP Protein, CYP7S1 Protein, cyp7a1a Protein, zgc:63920 Protein, cytochrome P450 family 7 subfamily A member 1 Protein, cytochrome P450, family 7, subfamily a, polypeptide 1 Protein, cytochrome P450, family 7, subfamily A, polypeptide 1 Protein, cholesterol 7-alpha-hydroxylase Protein, CYP7A1 Protein, Cyp7a1 Protein, cyp7a1 Protein

Background: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis.

Molecular Weight: 57.5 kDa

NCBI Accession: NP_000771

Pathways: Steroid Hormone Biosynthesis, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha