Calpastatin Protein (CAST) (Transcript Variant 10) (Myc-DYKDDDDK Tag)
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- Target See all Calpastatin (CAST) Proteins
- Calpastatin (CAST)
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 10
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Calpastatin protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Calpastatin (CAST), transcript variant 10 (transcript variant 10) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product CAST Protein
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Calpastatin (CAST) (AA 1-686) protein (GST tag)
CAST Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Calpastatin (CAST) (AA 582-669) protein (GST tag)CAST Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Calpastatin (CAST) (AA 1-708) protein (Strep Tag)Crystallography grade CAST Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Calpastatin (CAST) (AA 1-788) protein (Strep Tag)Crystallography grade CAST Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Calpastatin (CAST) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CAST Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Calpastatin (CAST) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)CAST Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Calpastatin (CAST)
- Abstract
- CAST Products
- Synonyms
- BS-17 Protein, calp3 Protein, CAST Protein, sb:cb966 Protein, zgc:194249 Protein, cast Protein, DKFZp469E0716 Protein, calpastatin Protein, calpastatin S homeolog Protein, Calpastatin Protein, CAST Protein, Cast Protein, cast.S Protein, cast Protein, VDBG_09182 Protein, ical Protein
- Background
- The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.
- Molecular Weight
- 71.7 kDa
- NCBI Accession
- NP_001035909
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