CA5B Protein (Myc-DYKDDDDK Tag)
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- Target See all CA5B Proteins
- CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CA5B protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Carbonic anhydrase 5B protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product CA5B Protein
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Carbonic Anhydrase VB, Mitochondrial (CA5B) (AA 1-317) protein (GST tag)
CA5B Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase VB, Mitochondrial (CA5B) (AA 2-90) protein (GST tag)CA5B Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase VB, Mitochondrial (CA5B) (AA 34-317) protein (His tag)Crystallography grade CA5B Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Carbonic Anhydrase VB, Mitochondrial (CA5B) (AA 34-317) protein (His tag)Crystallography grade CA5B Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Carbonic Anhydrase VB, Mitochondrial (CA5B) proteinCA5B Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))
- Alternative Name
- Carbonic Anhydrase 5b (CA5B Products)
- Synonyms
- CA-VB Protein, 7330410H16Rik Protein, CAVB Protein, Ca5b Protein, CarVb Protein, D730005F19Rik Protein, zgc:171653 Protein, CA5B Protein, ca5b Protein, carbonic anhydrase 5B Protein, carbonic anhydrase 5b, mitochondrial Protein, carbonic anhydrase Va Protein, carbonic anhydrase 5A Protein, carbonic anhydrase 5B, mitochondrial Protein, carbonic anhydrase VB, mitochondrial L homeolog Protein, CA5B Protein, Car5b Protein, Ca5b Protein, ca5a Protein, CA5A Protein, LOC100051095 Protein, ca5b.L Protein
- Background
- Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles.
- Molecular Weight
- 32.5 kDa
- NCBI Accession
- NP_009151
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