TNFSF8 Protein (His tag)
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- Target See all TNFSF8 Proteins
- TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- CHO Cells
- Purification tag / Conjugate
- This TNFSF8 protein is labelled with His tag.
- Application
- Functional Studies (Func), Protein Interaction (PI), Standard (STD), Antibody Production (AbP)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human CD153 / CD30L protein expressed in CHO cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by its ability to stimulate human IL-8 production by human PBMC using a concentration range of 10.0-25.0 ng/ml. Note: Results may vary with PBMC donors.
- Top Product
- Discover our top product TNFSF8 Protein
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Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) (AA 63-234) protein (mFc Tag)
TNFSF8 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC,
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) (AA 63-234) protein (His tag)TNFSF8 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) protein (mFc-His Tag)TNFSF8 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) (AA 63-234) protein (His-DYKDDDDK-Strep II Tag)TNFSF8 Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) (AA 63-234) (Active) protein (His tag)TNFSF8 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) (AA 1-234) protein (GST tag)TNFSF8 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) (AA 153-234) protein (GST tag)TNFSF8 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) proteinTNFSF8 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) (AA 63-234) protein (His tag)Crystallography grade TNFSF8 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) (AA 68-239) protein (His tag)Crystallography grade TNFSF8 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
- Alternative Name
- Cd153,cd30l (TNFSF8 Products)
- Synonyms
- CD153 Protein, CD30LG Protein, Cd30l Protein, CD30L Protein, TNFRSF8 Protein, TNFSF8 Protein, tumor necrosis factor (ligand) superfamily, member 8 Protein, TNF superfamily member 8 Protein, tumor necrosis factor superfamily member 8 Protein, Tnfsf8 Protein, TNFSF8 Protein
- Background
- Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. [UniProtKB/Swiss-Prot Function]
- Molecular Weight
- 21 kDa
- NCBI Accession
- NP_001235
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