CENPH Protein (Myc-DYKDDDDK Tag)
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- Target See all CENPH Proteins
- CENPH (Centromere Protein H (CENPH))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CENPH protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human CENP-H protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product CENPH Protein
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Centromere Protein H (CENPH) (AA 136-247) protein (His tag)
CENPH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
Centromere Protein H (CENPH) (AA 1-247) protein (GST tag)CENPH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Centromere Protein H (CENPH) (AA 1-241) protein (Strep Tag)Crystallography grade CENPH Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- CENPH (Centromere Protein H (CENPH))
- Alternative Name
- Cenp-H (CENPH Products)
- Synonyms
- 1700021I11Rik Protein, 2410018A12Rik Protein, 2610042E16Rik Protein, 2810046K12Rik Protein, AU044255 Protein, CENP-H Protein, ENP Protein, si:dkey-186m8.3 Protein, cenp-h Protein, centromere protein H Protein, centromere protein H pseudogene Protein, CENPH Protein, Cenph Protein, cenph Protein, LOC100356112 Protein, LOC101788801 Protein
- Background
- Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.
- Molecular Weight
- 28.3 kDa
- NCBI Accession
- NP_075060
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