CHFR Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2713969

Product Details

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CHFR protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human CHFR / RNF196 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Alternative Name: Chfr,rnf196 (CHFR Products)

Synonyms: RNF116 Protein, RNF196 Protein, CHFR Protein, fc43f10 Protein, si:dkey-69h6.7 Protein, wu:fc43f10 Protein, rnf116 Protein, rnf196 Protein, 5730484M20Rik Protein, C230082M18 Protein, checkpoint with forkhead and ring finger domains Protein, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase Protein, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase L homeolog Protein, CHFR Protein, chfr Protein, Chfr Protein, chfr.L Protein

Background: This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described.

Molecular Weight: 73.2 kDa

NCBI Accession: NP_001154816