ALDOB Protein (His tag)

Catalog No. ABIN2714560

Product Details

Target: ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ALDOB protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Aldolase B / ALDOB (full length, N-term HIS tag) protein expressed in E.coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))

Alternative Name: Aldolase B,aldob (ALDOB Products)

Synonyms: aldb Protein, aldo2 Protein, xaldb Protein, ALDOB Protein, aldob Protein, ALDB Protein, ALDO2 Protein, Aldo-2 Protein, Aldo2 Protein, BC016435 Protein, LIV10 Protein, fb25f09 Protein, wu:fb25f09 Protein, aldolase B Protein, aldolase, fructose-bisphosphate B Protein, aldolase, fructose-bisphosphate B S homeolog Protein, aldolase b, fructose-bisphosphate Protein, aldolase B, fructose-bisphosphate Protein, xaldb Protein, aldob Protein, aldob.S Protein, ALDOB Protein, Aldob Protein

Background: Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5 % of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.

Molecular Weight: 39.3 kDa

NCBI Accession: NP_000026