ACER1 Protein (Myc-DYKDDDDK Tag)
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- Target See all ACER1 Proteins
- ACER1 (Alkaline Ceramidase 1 (ACER1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ACER1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Alkaline ceramidase 1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product ACER1 Protein
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Alkaline Ceramidase 1 (ACER1) (AA 1-264) protein (Strep Tag)
Crystallography grade ACER1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Alkaline Ceramidase 1 (ACER1) (AA 1-273) protein (Strep Tag)Crystallography grade ACER1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- ACER1 (Alkaline Ceramidase 1 (ACER1))
- Alternative Name
- Alkaline Ceramidase 1 (ACER1 Products)
- Synonyms
- ASAH3 Protein, asah3 Protein, zgc:110285 Protein, ALKCDase1 Protein, 2310024P18Rik Protein, AI662009 Protein, Alkcdase1 Protein, Asah3 Protein, Cer1 Protein, alkaline ceramidase 1 Protein, ACER1 Protein, acer1 Protein, Acer1 Protein
- Background
- Ceramides are synthesized during epidermal differentiation and accumulate within the interstices of the stratum corneum, where they represent critical components of the epidermal permeability barrier. Excess cellular ceramide can trigger antimitogenic signals and induce apoptosis, and the ceramide metabolites sphingosine and sphingosine-1-phosphate (S1P) are important bioregulatory molecules. Ceramide hydrolysis in the nucleated cell layers regulates keratinocyte proliferation and apoptosis in response to external stress. Ceramide hydrolysis also occurs at the stratum corneum, releasing free sphingoid base that functions as an endogenous antimicrobial agent. ACER1 is highly expressed in epidermis and catalyzes the hydrolysis of very long chain ceramides to generate sphingosine (Houben et al., 2006 [PubMed 16477081] Sun et al., 2008 [PubMed 17713573]).[supplied by OMIM, Jul 2010].
- Molecular Weight
- 30.9 kDa
- NCBI Accession
- NP_597999
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