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PLAP Protein (His tag)

ALPP Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Catalog No. ABIN2714574
  • Target See all PLAP (ALPP) Proteins
    PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
    Protein Type
    Recombinant
    Origin
    • 9
    • 1
    • 1
    • 1
    Human
    Source
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This PLAP protein is labelled with His tag.
    Application
    Antibody Production (AbP), Standard (STD)
    Characteristics
    • Recombinant human Alkaline phosphatase / PLAP / ALPP protein expressed in HEK293 cells.
    • Produced with end-sequenced ORF clone
    Purity
    > 95 % as determined by SDS-PAGE and Coomassie blue staining
    Endotoxin Level
    Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
    Top Product
    Discover our top product ALPP Protein
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Comment

    The tag is located at the C-terminal.

    Restrictions
    For Research Use only
  • Buffer
    Supplied as a 0.2 μM filtered solution of20 mM TrisHCl,150 mM NaCl,0.2 mM GaCl,10 % Glycerol, pH 7.5
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
    Alternative Name
    Alkaline Phosphatase,plap,alpp (ALPP Products)
    Synonyms
    ECK0378 Protein, JW0374 Protein, psiA Protein, ALP Protein, PALP Protein, PLAP Protein, PLAP-1 Protein, ILC Protein, CTACK Protein, ESkine Protein, Akp2 Protein, alp Protein, zgc:56672 Protein, DDBDRAFT_0205491 Protein, DDBDRAFT_0231570 Protein, DDB_0205491 Protein, DDB_0231570 Protein, ALPI Protein, Actn2lp Protein, Alp Protein, bacterial alkaline phosphatase Protein, alkaline phosphatase, placental Protein, alkaline phosphatase, placental type Protein, C-C motif chemokine ligand 27 Protein, alkaline phosphatase, liver/bone/kidney Protein, alkaline phosphatase Protein, alkaline phosphatase, intestinal Protein, PDZ and LIM domain 3 Protein, phoA Protein, ALPP Protein, LOC100436725 Protein, CCL27 Protein, alpl Protein, alp Protein, ALPI Protein, Pdlim3 Protein, Alpp Protein
    Background
    The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.
    Molecular Weight
    53.7kD
    NCBI Accession
    NP_001623
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