PLAP Protein (His tag)
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- Target See all PLAP (ALPP) Proteins
- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PLAP protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Alkaline phosphatase / PLAP / ALPP protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Top Product
- Discover our top product ALPP Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Buffer
- Supplied as a 0.2 μM filtered solution of20 mM TrisHCl,150 mM NaCl,0.2 mM GaCl,10 % Glycerol, pH 7.5
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
- Alternative Name
- Alkaline Phosphatase,plap,alpp (ALPP Products)
- Synonyms
- ECK0378 Protein, JW0374 Protein, psiA Protein, ALP Protein, PALP Protein, PLAP Protein, PLAP-1 Protein, ILC Protein, CTACK Protein, ESkine Protein, Akp2 Protein, alp Protein, zgc:56672 Protein, DDBDRAFT_0205491 Protein, DDBDRAFT_0231570 Protein, DDB_0205491 Protein, DDB_0231570 Protein, ALPI Protein, Actn2lp Protein, Alp Protein, bacterial alkaline phosphatase Protein, alkaline phosphatase, placental Protein, alkaline phosphatase, placental type Protein, C-C motif chemokine ligand 27 Protein, alkaline phosphatase, liver/bone/kidney Protein, alkaline phosphatase Protein, alkaline phosphatase, intestinal Protein, PDZ and LIM domain 3 Protein, phoA Protein, ALPP Protein, LOC100436725 Protein, CCL27 Protein, alpl Protein, alp Protein, ALPI Protein, Pdlim3 Protein, Alpp Protein
- Background
- The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.
- Molecular Weight
- 53.7kD
- NCBI Accession
- NP_001623
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