alpha Actinin 4 Protein (Myc-DYKDDDDK Tag)
(1 reference)-
- Target See all alpha Actinin 4 (ACTN4) Proteins
- alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This alpha Actinin 4 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Alpha-actinin-4 / ACTN4 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product ACTN4 Protein
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Actinin, alpha 4 (ACTN4) (AA 1-911) protein (GST tag)
ACTN4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Actinin, alpha 4 (ACTN4) (AA 397-648) protein (His tag)ACTN4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Actinin, alpha 4 (ACTN4) (AA 592-701) protein (GST tag)ACTN4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Actinin, alpha 4 (ACTN4) (AA 1-911) protein (Strep Tag)Crystallography grade ACTN4 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Actinin, alpha 4 (ACTN4) (AA 1-912) protein (Strep Tag)Crystallography grade ACTN4 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "Alpha-Actinin-4 is a Possible Target Protein for Aristolochic Acid I in Human Kidney Cells In Vitro." in: The American journal of Chinese medicine, Vol. 44, Issue 2, pp. 291-304, (2016) (PubMed).
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: "Alpha-Actinin-4 is a Possible Target Protein for Aristolochic Acid I in Human Kidney Cells In Vitro." in: The American journal of Chinese medicine, Vol. 44, Issue 2, pp. 291-304, (2016) (PubMed).
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- Target
- alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))
- Alternative Name
- alpha-Actinin-4,actn4 (ACTN4 Products)
- Synonyms
- fsgs Protein, fsgs1 Protein, C77391 Protein, ACTININ-4 Protein, FSGS Protein, FSGS1 Protein, wu:fb53f05 Protein, zgc:63508 Protein, actinin alpha 4 Protein, actinin, alpha 4 Protein, actinin alpha 4 S homeolog Protein, actn4 Protein, ACTN4 Protein, Actn4 Protein, actn4.S Protein
- Background
- Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
- Molecular Weight
- 104.7 kDa
- NCBI Accession
- NP_004915
- Pathways
- Proton Transport
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