Annexin VII Protein (Transcript Variant 1) (His tag)

Catalog No. ABIN2714748

Product Details

Target: Annexin VII (ANXA7) (Annexin A7 (ANXA7))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Annexin VII protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Annexin A7 / ANXA7 (transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: < 0.1 EU per μg protein as determined by LAL test

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 10 mM Tris-HCl, 100 mM NaCl, pH 8.0. Stable for at least 6 months from date of receipt under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Annexin VII (ANXA7) (Annexin A7 (ANXA7))

Alternative Name: Annexin a7,anxa7 (ANXA7 Products)

Synonyms: ANX7 Protein, SNX Protein, SYNEXIN Protein, AI265384 Protein, AI316497 Protein, Anx7 Protein, synexin Protein, anxa7 Protein, MGC76267 Protein, MGC82023 Protein, ANXA7 Protein, MGC83033 Protein, annexin A7 Protein, annexin VII Protein, annexin A7 S homeolog Protein, ANXA7 Protein, Anxa7 Protein, anxa7 Protein, anxa7.S Protein, PTRG_02166 Protein, BRAFLDRAFT_231082 Protein, PAAG_00136 Protein, MCYG_04180 Protein, VDBG_06328 Protein, MGYG_02426 Protein

Background: Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion.

Molecular Weight: 50.3 kDa

NCBI Accession: NP_001147