APEX2 Protein (Myc-DYKDDDDK Tag)
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- Target See all APEX2 Proteins
- APEX2 (APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This APEX2 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human APEX2 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product APEX2 Protein
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APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2) (AA 1-518) protein (Strep Tag)
Crystallography grade APEX2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2) (AA 1-516) protein (Strep Tag)Crystallography grade APEX2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- APEX2 (APEX Nuclease (Apurinic/apyrimidinic Endonuclease) 2 (APEX2))
- Alternative Name
- Apex2 (APEX2 Products)
- Synonyms
- APE2 Protein, APEXL2 Protein, XTH2 Protein, ape2 Protein, apexl2 Protein, xth2 Protein, zgc:55889 Protein, C430040P13Rik Protein, apurinic/apyrimidinic endodeoxyribonuclease 2 Protein, APEX nuclease (apurinic/apyrimidinic endonuclease) 2 Protein, apurinic/apyrimidinic endodeoxyribonuclease 2 L homeolog Protein, apurinic/apyrimidinic endonuclease 2 Protein, APEX2 Protein, apex2 Protein, apex2.L Protein, Apex2 Protein
- Background
- Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
- Molecular Weight
- 57.2 kDa
- NCBI Accession
- NP_055296
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