ATP6V1G3i Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2715064

Product Details

Target: ATP6V1G3i (ATP6V1G3) (ATPase, H+ Transporting, Lysosomal 13kDa, V1 Subunit G3 (ATP6V1G3))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 2

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ATP6V1G3i protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human ATP6V1G3 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ATP6V1G3i (ATP6V1G3) (ATPase, H+ Transporting, Lysosomal 13kDa, V1 Subunit G3 (ATP6V1G3))

Alternative Name: Atp6v1g3 (ATP6V1G3 Products)

Synonyms: ATP6G3 Protein, Vma10 Protein, ATPase H+ transporting V1 subunit G3 Protein, ATPase, H+ transporting, lysosomal V1 subunit G3 Protein, ATP6V1G3 Protein, Atp6v1g3 Protein

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'' and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three G subunit proteins. Transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 6.7 kDa

NCBI Accession: NP_579872

Pathways: Transition Metal Ion Homeostasis, Proton Transport