BCAT1 Protein (His tag)
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- Target See all BCAT1 Proteins
- BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This BCAT1 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human BCAT1 (full length, N-term HIS tag, transcript variant 1) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product BCAT1 Protein
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Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1) (AA 1-386) protein (His tag)
BCAT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1) (AA 1-320) protein (GST tag)BCAT1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1) (AA 1-386) protein (Strep Tag)Crystallography grade BCAT1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1) (AA 1-386) protein (Strep Tag)Crystallography grade BCAT1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1) protein (Myc-DYKDDDDK Tag)BCAT1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))
- Alternative Name
- Bcat1 (BCAT1 Products)
- Synonyms
- fj66g02 Protein, zgc:73157 Protein, wu:fj66g02 Protein, Bcatc Protein, BCATC Protein, BCT1 Protein, ECA39 Protein, MECA39 Protein, PNAS121 Protein, PP18 Protein, BCATc Protein, Eca39 Protein, branched chain amino-acid transaminase 1, cytosolic Protein, branched chain amino acid transaminase 1 Protein, branched chain amino-acid transaminase 1, cytosolic L homeolog Protein, branched chain aminotransferase 1, cytosolic Protein, bcat1 Protein, BCAT1 Protein, bcat1.L Protein, Bcat1 Protein
- Background
- This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described.
- Molecular Weight
- 42.8 kDa
- NCBI Accession
- NP_005495
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