BUB1 Protein (Myc-DYKDDDDK Tag)
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- Target See all BUB1 Proteins
- BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This BUB1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human BUB1A protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product BUB1 Protein
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Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1) (AA 1-1085) protein (GST tag)
BUB1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1) (AA 1-130) protein (GST tag)BUB1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1) (AA 1-1085) protein (Strep Tag)Crystallography grade BUB1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1) (AA 1-1058) protein (Strep Tag)Crystallography grade BUB1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))
- Alternative Name
- Bub1a (BUB1 Products)
- Synonyms
- bub1a Protein, bub1l Protein, hbub1 Protein, xbub1 Protein, BUB1A Protein, BUB1L Protein, hBUB1 Protein, AL022991 Protein, Bub1a Protein, C80208 Protein, D2Xrf87 Protein, BUB1 mitotic checkpoint serine/threonine kinase Protein, BUB1, mitotic checkpoint serine/threonine kinase Protein, BUB1 Protein, bub1 Protein, Bub1 Protein
- Background
- This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.
- Molecular Weight
- 122.2 kDa
- NCBI Accession
- NP_004327
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