C11orf73 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2715388

Product Details

Target: C11orf73 (Chromosome 11 Open Reading Frame 73 (C11orf73))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This C11orf73 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human C11orf73 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: C11orf73 (Chromosome 11 Open Reading Frame 73 (C11orf73))

Alternative Name: c11orf73 (C11orf73 Products)

Synonyms: C11orf73 Protein, HSPC179 Protein, Hikeshi Protein, L7RN6 Protein, OPI10 Protein, Hikeshi, heat shock protein nuclear import factor Protein, Hikeshi, heat shock protein nuclear import factor L homeolog Protein, HIKESHI Protein, hikeshi.L Protein, hikeshi Protein, Hikeshi Protein

Background: This gene encodes an evolutionarily conserved nuclear transport receptor that mediates heat-shock-induced nuclear import of 70 kDa heat-shock proteins (Hsp70s) through interactions with FG-nucleoporins. The protein mediates transport of the ATP form but not the ADP form of Hsp70 proteins under conditions of heat shock stress. Structural analyses demonstrate that the protein forms an asymmetric homodimer and that the N-terminal domain consists of a jelly-roll/beta-sandwich fold structure that contains hydrophobic pockets involved in FG-nucleoporin recognition. Reduction of RNA expression levels in HeLa cells using small interfering RNAs results in inhibition of heat shock-induced nuclear accumulation of Hsp70s, indicating a role for this gene in regulation of Hsp70 nuclear import during heat shock stress.

Molecular Weight: 21.4 kDa

NCBI Accession: NP_057485

Pathways: Protein targeting to Nucleus