C12orf65 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- Target See all C12orf65 Proteins
- C12orf65 (Chromosome 12 Open Reading Frame 65 (C12orf65))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This C12orf65 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human C12orf65 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product C12orf65 Protein
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Chromosome 12 Open Reading Frame 65 (C12orf65) (AA 1-166) protein (GST tag)
C12orf65 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Chromosome 12 Open Reading Frame 65 (C12orf65) (AA 36-166) protein (His tag)Crystallography grade C12orf65 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- C12orf65 (Chromosome 12 Open Reading Frame 65 (C12orf65))
- Alternative Name
- c12orf65 (C12orf65 Products)
- Synonyms
- COXPD7 Protein, SPG55 Protein, chromosome 12 open reading frame 65 Protein, C12orf65 Protein
- Background
- This nuclear gene encodes a mitochondrial matrix protein that appears to contribute to peptide chain termination in the mitochondrial translation machinery. Two different 1 bp deletions (resulting in the same premature stop codon)result in decreased mitochondrial translation, decreased levels of oxidative phosphorylation complexes and encepthalomyopathy. Alternative splicing results in multiple transcript variants.
- Molecular Weight
- 18.6 kDa
- NCBI Accession
- NP_689482
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