DBT Protein (Myc-DYKDDDDK Tag)
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- Target See all DBT Proteins
- DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DBT protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human DBT protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product DBT Protein
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Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (AA 62-482) protein (His tag)
Crystallography grade DBT Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, Crys, SDS, WB
Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (AA 62-482) protein (His tag)Crystallography grade DBT Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, Crys, SDS, WB
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))
- Alternative Name
- Dbt (DBT Products)
- Synonyms
- BCATE2 Protein, BCKAD-E2 Protein, BCKADE2 Protein, E2 Protein, E2B Protein, D3Wsu60e Protein, im:7147214 Protein, zgc:103768 Protein, E2b Protein, dihydrolipoamide branched chain transacylase E2 Protein, hypothetical protein Protein, dihydrolipoamide branched chain transacylase E2 L homeolog Protein, DBT Protein, dbt Protein, CpipJ_CPIJ006326 Protein, BDBG_05874 Protein, NAEGRDRAFT_78509 Protein, VDBG_04820 Protein, PGTG_17722 Protein, Dbt Protein, dbt.L Protein
- Target Type
- Viral Protein
- Background
- The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component. [UniProtKB/Swiss-Prot Function]
- Molecular Weight
- 46.4 kDa
- NCBI Accession
- NP_001909
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