DDX58 Protein (Myc-DYKDDDDK Tag)
(1 reference)-
- Target See all DDX58 Proteins
- DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DDX58 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human DDX58 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product DDX58 Protein
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DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 1-925) protein (GST tag)
DDX58 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 1-925) protein (Strep Tag)Crystallography grade DDX58 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 1-926) protein (Strep Tag)Crystallography grade DDX58 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 1-430), (ATP-binding domain) protein (His tag)DDX58 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) proteinDDX58 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "Quantitation of the dynamic profiles of the innate immune response using multiplex selected reaction monitoring-mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 6, pp. 1513-29, (2013) (PubMed).
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: "Quantitation of the dynamic profiles of the innate immune response using multiplex selected reaction monitoring-mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 6, pp. 1513-29, (2013) (PubMed).
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- Target
- DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))
- Alternative Name
- Ddx58 (DDX58 Products)
- Synonyms
- RIG-I Protein, RIGI Protein, RLR-1 Protein, 6430573D20Rik Protein, C330021E21 Protein, RHIV-1 Protein, RIG-1 Protein, DExD/H-box helicase 58 Protein, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 Protein, DEXD/H-box helicase 58 Protein, DDX58 Protein, Ddx58 Protein
- Background
- DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response.
- Molecular Weight
- 106.4 kDa
- NCBI Accession
- NP_055129
- Pathways
- Activation of Innate immune Response, Hepatitis C
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