Dematin Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719380

Product Details

Target: Dematin (EPB49) (erythrocyte Membrane Protein Band 4.9 (Dematin) (EPB49))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 2

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Dematin protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Dematin (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Dematin (EPB49) (erythrocyte Membrane Protein Band 4.9 (Dematin) (EPB49))

Alternative Name: Dematin (EPB49 Products)

Synonyms: MGC80597 Protein, MGC108072 Protein, EPB49 Protein, DMT Protein, AI325486 Protein, Epb4.9 Protein, Epb49 Protein, dematin Protein, dematin actin binding protein L homeolog Protein, dematin actin binding protein Protein, dmtn.L Protein, DMTN Protein, dmtn Protein, Dmtn Protein

Background: The protein encoded by this gene is an actin binding and bundling protein that plays a structural role in erythrocytes, by stabilizing and attaching the spectrin/actin cytoskeleton to the erythrocyte membrane in a phosphorylation-dependent manner. This protein contains a core domain in the N-terminus, and a headpiece domain in the C-terminus that binds F-actin. When purified from erythrocytes, this protein exists as a trimer composed of two 48 kDa polypeptides and a 52 kDa polypeptide. The different subunits arise from alternative splicing in the 3&apos coding region, where the headpiece domain is located. Disruption of this gene has been correlated with the autosomal dominant Marie Unna hereditary hypotrichosis disease, while loss of heterozygosity of this gene is thought to play a role in prostate cancer progression. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 45.3 kDa

NCBI Accession: NP_001107607

Pathways: Regulation of Actin Filament Polymerization